ZO-1 Rabbit pAb for WB, IF/ICC, ELISA - Q07157

Core Product Specifications and Parameters

Biological Background: ZO-1 Function and Localization

• ZO-1 (TJP1) is a member of the zonula occludens protein family, which also includes TJP2 and TJP3; these scaffolding proteins link transmembrane tight junction proteins (e.g., claudins) to the actin cytoskeleton. Related references: PMID:7798316, PMID:9792688
• It is essential for the formation of tight junction belts that act as barriers to paracellular diffusion and maintain apical-basolateral polarity in epithelial and endothelial cells.
• ZO-1 is involved in the regulation of cell migration by targeting CDC42BPB to the leading edge of migrating cells. Related references: PMID:21240187
• Plays a role in podosome formation and function, thereby regulating cell adhesion and matrix remodeling. Related references: PMID:20930113
• Subcellularly, ZO-1 localizes to the cell membrane at tight junctions, gap junctions, and podosome structures.
• Tissue-specific isoforms exist: the alpha-containing isoform is predominantly expressed in most epithelial cell junctions, while a short isoform is found in endothelial cells and specialized junctions of renal glomeruli and Sertoli cells.
• Post-translational modifications include phosphorylation, and the protein contains calmodulin-binding and SH3 domains, as indicated by UniProt keywords.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 18-273 of human TJP1, targeting the N-terminal region; consider this when working with truncated constructs.
• For Western blotting, expect a band at ~195 kDa; optimize transfer conditions for high molecular weight proteins.
• In immunofluorescence/ICC, tight junction staining may require permeabilization; validate fixation and permeabilization protocols for your specific cell type.
• Cross-reactivity with mouse and rat enables use in rodent models; verify reactivity in your system if species-specific differences are a concern.
• Not listed for IP or IHC; if required, perform pilot experiments to assess suitability.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research labs with one-stop access to high-quality IVD raw materials, including this ZO-1 antibody for tight junction and epithelial barrier research. Our supply capabilities span validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and sourcing of rare target raw materials. We are dedicated to supporting your development from concept to clinic with reliable procurement solutions.