ZNF444 Rabbit pAb - Q8N0Y2

Core Product Specifications and Parameters

Biological Background: ZNF444 Function and Localization

• Zinc finger protein 444 (ZNF444), also known as endothelial zinc finger protein 2 (EZF-2) or zinc finger and SCAN domain-containing protein 17, is encoded by the ZNF444 gene (alternative splicing produces multiple isoforms).
• It functions as a transcriptional regulator, specifically binding to the 5'-flanking critical region of the SCARF1 (scavenger receptor class F member 1) promoter.
• The protein contains a SCAN domain and multiple C2H2-type zinc fingers, which mediate DNA binding and protein-protein interactions.
• Subcellularly, ZNF444 is localized to the nucleus, consistent with its role in transcription regulation.
• Post-translational modifications include acetylation, phosphorylation, and ubiquitination (isopeptide bond formation), suggesting dynamic regulation of its activity and stability.
• Keywords such as "Alternative splicing," "DNA-binding," "Metal-binding," "Isopeptide bond," "Ubl conjugation," and "Zinc-finger" highlight its structural and functional features.

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 1–327 of human ZNF444, covering the N-terminal region; consider that this antibody may detect all known isoforms if the epitope is conserved.
• For western blot (WB), expect a band at approximately 35 kDa; validate in the relevant cell or tissue lysate, as reactivity has been demonstrated in human, mouse, and rat samples.
• When performing immunohistochemistry (IHC-P), include proper antigen retrieval steps and negative controls to confirm specificity.
• For immunofluorescence (IF/ICC), optimize fixation and permeabilization conditions based on your cell type.
• In ELISA applications, titrate the antibody and coating antigen to establish optimal signal-to-noise ratios.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with end-to-end access to IVD raw materials, technical services, and consulting, accelerating development from concept to clinic. We offer validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and custom sourcing for targets like ZNF444 to support transcriptional regulation research. Our integrated supply chain ensures reliable, research-grade materials for your assay development and validation needs.