ZNF24 Rabbit PolymAb® Monoclonal Antibody for WB, IF/ICC, IF-P, IHC-P, ELISA - P17028

Core Product Specifications and Parameters

Biological Background: ZNF24 Function and Localization

• ZNF24 (Zinc finger protein 24) is a transcription factor also designated KOX17, RSG-A (Retinoic acid suppression protein A), ZNF191, ZSCAN3, and Zfp191.
• It plays an essential role in oligodendrocyte myelination in the central nervous system, driving the transition from the premyelinating to myelinating state and maintaining progenitor cell proliferation (By similarity).
• ZNF24 acts as a transcriptional repressor in vitro and binds specifically to the 5'-TCAT-3' DNA motif.
• The protein localizes to the nucleus.
• Post-translational modifications include phosphorylation (Phosphoprotein keyword) and isopeptide bond/ubl conjugation, suggesting ubiquitination-like regulation.
• ZNF24 is broadly expressed across human tissues, with the notable exception of the heart.
• Alternative splicing generates multiple isoforms (Alternative splicing keyword).
• It belongs to the large zinc finger family, characterized by SCAN and zinc finger domains.

Experimental Guidance and Technical Tips

• For Western blot (WB), the expected molecular weight of ZNF24 is approximately 42 kDa; validate the band size in your specific experimental system.
• The immunogen (amino acids 160-242) targets a region within the protein, potentially recognizing multiple isoforms; confirm through appropriate controls.
• For immunofluorescence (IF/ICC, IF-P) and immunohistochemistry (IHC-P), nuclear staining is anticipated; optimize fixation and permeabilization conditions.
• Cross-reactivity with mouse and rat ZNF24 is reported, but species-specific validation is recommended before quantitative assays.
• ELISA applications may be suitable for detecting the immunogen or recombinant protein; assess linearity and sensitivity using purified protein standards.
• Consider testing the antibody at multiple dilutions to determine optimal working concentrations for your specific application and model.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides a comprehensive one-stop sourcing solution for IVD raw materials, from validated antibody pairs and optimized monoclonal/polyclonal antibodies to bulk ancillary reagents and rare target sourcing. The ZNF24 transcription factor is a key target in neurobiology and transcriptional regulation research, and CamelBio can support your projects with tailored antibody supply and technical expertise spanning concept-to-clinic development.