ZNF148 Rabbit pAb - Q9UQR1

Core Product Specifications and Parameters

Biological Background: ZNF148 Function and Localization

• ZNF148, also known as ZBP-89 and ZFP148, is a zinc-finger transcription factor that primarily functions as a transcriptional repressor.
• It represses the transcription of several genes, including gastrin, stromelysin, and enolase, by binding to G-rich box sequences in their enhancer regions.
• The protein contains multiple zinc-finger domains that mediate sequence-specific DNA binding, a hallmark of its regulatory mechanism.
• ZNF148 is localized exclusively in the nucleus, consistent with its role in modulating gene expression.
• Post-translational modifications such as acetylation, phosphorylation, and ubiquitination have been identified, potentially influencing its stability and activity.
• Alternative splicing generates multiple isoforms, expanding the functional diversity of the protein.
• Genetic variations and dysregulation of ZNF148 have been linked to developmental disorders, including dwarfism and intellectual disability, underscoring its biological significance.

Experimental Guidance and Technical Tips

• The immunogen (amino acids 690–794) covers the C-terminal region rich in zinc-finger motifs; validate specificity using ZNF148 knockout or knockdown samples.
• For Western blot, expect a band near 89 kDa; consider using nuclear extracts from human, mouse, or rat cell lines for optimal detection.
• In IHC-P and IF/ICC, nuclear staining is anticipated; optimize antigen retrieval and permeabilization steps accordingly.
• Although cross-reactivity is reported for human, mouse, and rat, independent validation in other species or tissues is recommended.
• For ELISA, use purified recombinant ZNF148 protein as a coating standard and confirm linearity with known positive controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a dedicated one-stop sourcing partner for IVD raw materials, supporting diagnostic manufacturers, research laboratories, and academic institutes worldwide. This polyclonal antibody against ZNF148 addresses transcription regulation research, with direct applications in oncology, developmental biology, and epigenetic studies. Through our sourcing network, we offer validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and assistance with rare-target raw material procurement to accelerate your assay development and production needs.