ZNF131 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - P52739

Core Product Specifications and Parameters

Biological Background: ZNF131 Function and Localization

• ZNF131, also known as ZBTB35 or pHZ-10, is a zinc finger protein belonging to the Krüppel C2H2-type zinc finger family. It contains multiple zinc finger domains that mediate DNA binding and transcriptional regulation.
• ZNF131 functions as a transcriptional repressor, with evidence indicating it represses ESR1/ER-alpha signaling. This suggests a role in modulating estrogen receptor-mediated transcription.
• The protein is involved in developmental processes and organogenesis, as well as in the function of the adult central nervous system (By similarity).
• ZNF131 localizes to the nucleus, consistent with its role as a transcription factor.
• Predominant expression of ZNF131 is observed in specific brain regions, including the occipital and temporal lobes, nucleus caudatus, hippocampus, and cerebellum, as well as in testis and thymus.
• Alternative splicing of ZNF131 generates multiple isoforms, which may contribute to functional diversity.
• Post-translational modifications include isopeptide bond formation and ubiquitination, indicating regulation by ubiquitin-like conjugation.
• The protein's zinc finger domains suggest it binds zinc ions, essential for its structural integrity and DNA-binding activity.

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 80-240, so the antibody may recognize multiple isoforms if the epitope is conserved. Consider validating specificity in your experimental system, for example by siRNA knockdown.
• For WB, the predicted molecular weight is ~71 kDa, but actual migration may differ; include positive controls from human, mouse, or rat tissues with known ZNF131 expression (e.g., brain, testis).
• In IF/ICC, expect nuclear localization; use a nuclear counterstain to confirm.
• Optimize antibody dilutions for each application; supplier-recommended concentrations may require titration for your specific conditions.
• Due to alternative splicing, multiple bands may appear in WB; verify with appropriate controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD raw materials, offering a streamlined bridge from concept to clinic for diagnostic manufacturers, laboratories, and research institutes. Our portfolio includes this ZNF131 antibody, relevant for neuroscience and transcription research, alongside validated antibody pairs, optimized polyclonal and monoclonal antibodies, bulk ancillary reagents, and custom sourcing for rare targets. Let CamelBio support your procurement needs with reliable access to the materials required for your diagnostic and research workflows.