ZEB1 Rabbit Monoclonal Antibody for WB, ELISA - P37275

Core Product Specifications and Parameters

Biological Background: ZEB1 Function and Localization

• ZEB1, also known as TCF8 or ZFHX1A, is a zinc finger homeobox transcription factor that functions primarily as a transcriptional repressor.
• It inhibits IL-2 gene expression and represses E-cadherin promoter, promoting epithelial-mesenchymal transition (EMT) by recruiting SMARCA4/BRG1.
• ZEB1 can enhance or repress ATP1A1 promoter depending on cell type and cDNA quantity, and represses BCL6 transcription in the presence of corepressor CTBP1.
• It positively regulates neuronal differentiation and represses RCOR1 transcription activation during neurogenesis.
• The protein localizes to the nucleus and colocalizes with SMARCA4/BRG1 in E-cadherin-negative cells and at the invasion front of colorectal carcinomas.
• ZEB1 is expressed in heart and skeletal muscle, but not in liver, spleen, or pancreas.
• Keywords highlight its roles in differentiation, neurogenesis, and disease variants associated with corneal dystrophy. Post-translational modifications include phosphorylation, ubiquitination, and isopeptide bond formation.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 800-900 of human ZEB1. Consider testing epitope accessibility under native versus denaturing conditions for optimal WB performance.
• For WB, the predicted molecular weight is 124 kDa. Validate in your specific sample system, as ZEB1 expression varies by cell type and EMT status.
• For ELISA, perform a titration curve to determine the optimal antibody concentration and coating conditions.
• Cross-reactivity is expected with human and rat; for other species, verify sequence homology of the immunogen region before use.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides a comprehensive range of IVD raw materials, including validated antibodies, antigens, and ancillary reagents, to support your assay development from concept to clinic. The ZEB1 antibody is part of our transcription factor and cancer research portfolio, ideal for studies on EMT and tumor progression. As your strategic partner, we also specialize in sourcing rare targets and offering custom monoclonal antibody development services to accelerate your diagnostic pipeline.