ZAP70 Rabbit Monoclonal Antibody for WB, IF-P, ELISA - P43403

Core Product Specifications and Parameters

Biological Background: ZAP70 Function and Localization

• Tyrosine-protein kinase ZAP-70 (70 kDa zeta-chain associated protein, Syk-related tyrosine kinase) is encoded by the ZAP70 gene and exists as alternatively spliced isoforms.
• Functions as an essential signaling kinase in the adaptive immune response: regulates T-cell activation, proliferation, differentiation, motility, adhesion, and cytokine expression; also contributes to thymocyte development and primary B-cell activation.
• Upon T-cell receptor (TCR) stimulation, ZAP70 is recruited to phosphorylated ITAM motifs of CD3E and CD3Z at the plasma membrane, where it is activated by LCK-mediated phosphorylation. Activated ZAP70 phosphorylates adaptor proteins LAT and LCP2, initiating downstream signaling cascades. Related references: PMID:7509083
• Mediates TCR complex internalization and degradation by interacting with the E3 ubiquitin ligase CBL and adaptors SLA/SLA2, modulating surface TCR expression and signaling duration.
• Subcellular localization: cytoplasm and cell membrane; translocates to the plasma membrane upon TCR engagement.
• Tissue specificity: expressed in T-cells, natural killer cells, early thymocytes, and pro/pre B-cells.
• Post-translational modifications: phosphorylation, acetylation, and ubiquitination regulate its activity and stability. Contains SH2 domains and a kinase domain. Keywords: 3D-structure, phosphoprotein, Ubl conjugation, isopeptide bond, disease variant, SCID.

Experimental Guidance and Technical Tips

• The immunogen is a recombinant fusion protein representing amino acids 550-619 within the C-terminal kinase domain of human ZAP70. For Western blot, expect a specific band around 70 kDa in human T-cell lines (e.g., Jurkat) or peripheral blood mononuclear cells (PBMCs).
• For immunofluorescence (IF-P), permeabilization is required to detect intracellular and membrane-associated ZAP70; consider using T-cell activators to enhance signal.
• In ELISA applications, validate antibody performance with recombinant ZAP70 protein or cell lysates from relevant T-cell samples; titrate optimally.
• Reported cross-reactivity is for human. If testing other species, verify sequence conservation of the immunogen region, particularly in the kinase domain.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides comprehensive IVD raw material sourcing for diagnostic developers and research institutes, covering immune profiling, lymphoma diagnostics, and T-cell signaling research. For ZAP70-targeted assays, we offer validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare-target raw material procurement. Our one-stop supply chain and technical services streamline your development from concept to clinic.