YY1 Rabbit pAb for WB, ELISA, ChIP - P25490

Core Product Specifications and Parameters

Biological Background: YY1 Function and Localization

• YY1 (Yin Yang 1), also known as Delta transcription factor, NF-E1, INO80S, and UCRBP, is a multifunctional transcription factor that exhibits both repressor and activator functions depending on the promoter context and interacting cofactors.
• It binds to the consensus DNA sequence 5'-CCGCCATNTT-3' near transcription start sites; methylation of the initial CG dinucleotide greatly reduces binding affinity. Related references: PMID:15329343 PMID:17721549 PMID:24326773
• In the absence of adenovirus E1A protein, YY1 acts as a repressor, but in its presence, it switches to an activator, demonstrating its context-dependent transcriptional regulation. Related references: PMID:1655281
• YY1 synergizes with SMAD1 and SMAD4 in bone morphogenetic protein (BMP)-mediated cardiac-specific gene expression by binding to SMAD binding elements within BMP response elements. Related references: PMID:15329343
• It recruits the PRC2/EED-EZH2 complex to mediate transcriptional repression and also interacts with the PR-DUB complex, targeting specific gene-regulatory regions for deubiquitination. Related references: PMID:11158321 PMID:20805357
• YY1 is proposed as a core component of the chromatin remodeling INO80 complex and is involved in DNA repair, in vitro binding to Holliday junction recombination intermediates. Related references: PMID:18026119 PMID:28575647
• Subcellularly, YY1 localizes to the nucleus matrix, consistent with its role in transcriptional regulation and chromatin organization.
• Post-translational modifications of YY1 include phosphorylation, ADP-ribosylation, and ubiquitination; the protein contains multiple zinc-finger domains essential for DNA binding.

Experimental Guidance and Technical Tips

• The immunogen targeting amino acids 80–160 of human YY1 may detect both full-length and truncated isoforms; validate the observed band pattern in your specific sample system.
• For Western blotting, the predicted molecular weight is ~45 kDa; optimize blocking conditions and antibody dilution using whole-cell lysates from human, mouse, or rat cells.
• In ChIP assays, include appropriate positive and negative control loci (e.g., known YY1 target promoters vs. intergenic regions) and confirm enrichment with qPCR.
• When using ELISA, consider cross-reactivity testing against other zinc-finger transcription factors if non-specific signals are observed.

CamelBio: Your One-Stop Sourcing Bridge

As a one-stop IVD raw-material sourcing partner, CamelBio connects diagnostic manufacturers and researchers with validated antibody pairs, optimized polyclonal and monoclonal antibodies, and bulk ancillary reagents. This YY1 antibody supports transcription regulation and chromatin research, and we provide customized sourcing for rare targets and technical services to accelerate your assay development from concept to clinic.