YTHDF2 Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - Q9Y5A9

Core Product Specifications and Parameters

Biological Background: YTHDF2 Function and Localization

• YTHDF2 is an N6-methyladenosine (m6A) reader protein that specifically binds m6A-containing RNAs, regulating their stability and processing. Related references: PMID:24284625, PMID:26046440, PMID:26318451

• Acts as a key regulator of mRNA decay, promoting degradation of m6A-modified transcripts via recruitment of the CCR4-NOT deadenylase complex or the ribonuclease P/MRP complex. Related references: PMID:24284625, PMID:26046440, PMID:27558897

• Functions redundantly with YTHDF1 and YTHDF3 to mediate m6A-dependent mRNA degradation and cellular differentiation. Related references: PMID:28106072, PMID:32492408

• Under heat shock stress, relocalizes to the nucleus and promotes cap-independent translation of m6A-marked mRNAs by protecting them from FTO-mediated demethylation. Related references: PMID:26458103

• Localizes to the cytosol, P-bodies, and stress granules; undergoes liquid-liquid phase separation upon binding to polymethylated mRNAs. Related references: PMID:31292544, PMID:31388144, PMID:31642031

• Regulates innate immunity by binding m6A-containing IFNB transcripts and promoting their degradation, thereby inhibiting type I interferon response. Related references: PMID:30559377

• Highly expressed in induced pluripotent stem cells and downregulated during neural differentiation; restrains neural specification by degrading methylated neural-specific mRNAs. Related references: PMID:32169943

• Involved in viral life cycles: promotes SV40 and KSHV gene expression and replication by binding m6A-containing viral RNAs. Related references: PMID:29447282, PMID:29659627

Experimental Guidance and Technical Tips

• The immunogen corresponds to a region within the YTH domain of human YTHDF2; antibody reactivity with mouse and rat orthologs has been observed, but validation in the specific model system is recommended.
• For Western blot, a band around 62 kDa is expected; consider using positive controls such as HeLa or HEK293T lysates.
• For IHC-P and IF/ICC, optimize antibody dilution and antigen retrieval conditions for your tissue or cell type, and validate with appropriate controls.
• This antibody is suitable for ELISA; pairing with a matched detection antibody may enable sandwich ELISA development.
• Note that YTHDF2 can localize to both cytoplasmic and nuclear compartments; subcellular staining patterns may vary with cell type and stress conditions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio partners with diagnostic manufacturers and research institutions, offering a comprehensive portfolio of IVD raw materials. This YTHDF2 polyclonal antibody is part of our growing epitranscriptomics toolset; we can supply validated antibody pairs, bulk quantities, and ancillary reagents to accelerate your assay development. Contact us for customized sourcing of rare-target antibodies, recombinant proteins, and expert technical consultation.