YTHDC2 Rabbit Polyclonal Antibody for WB, ELISA - Q9H6S0

Core Product Specifications and Parameters

Biological Background: YTHDC2 Function and Localization

• 3'-5' RNA helicase that plays a key role in the male and female germline by promoting the transition from mitotic to meiotic divisions in stem cells. Related references: PMID:26318451, PMID:29033321, PMID:29970596
• Specifically recognizes and binds N6-methyladenosine (m6A)-containing RNAs, promoting degradation of m6A-containing mRNAs through its helicase activity, possibly mediated by XRN1 exoribonuclease. Related references: PMID:26318451, PMID:29033321
• Required for both spermatogenesis and oogenesis (By similarity).
• Subcellular localization is cytoplasmic, with enrichment in the perinuclear region.
• Expressed in testis, specifically up-regulated in spermatocytes upon entry into meiosis. Related references: PMID:29087293
• Contains ANK repeats and an ATP-binding domain; annotated with keywords including helicase, hydrolase, meiosis, oogenesis, phosphoprotein, RNA-binding, and spermatogenesis. Predicted molecular weight of approximately 160 kDa.

Experimental Guidance and Technical Tips

• For Western blotting, a band of approximately 160 kDa is expected; validate using lysates from YTHDC2-expressing tissues or cell lines such as testis.
• Consider species-specific reactivity: this antibody is reported to react with human, mouse, and rat, so verify cross-reactivity in your sample system.
• In ELISA applications, the antibody may be useful as a capture or detection reagent; titrate appropriately for your specific assay format.
• As a polyclonal antibody, lot-to-lot consistency may vary; include positive and negative controls and consider performing a specificity validation.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a one-stop sourcing partner providing diagnostic manufacturers, laboratories, and research institutes with access to IVD raw materials, technical services, and consulting—from concept to clinic. For YTHDC2 and germline/RNA modification research, we offer validated antibody pairs, optimized polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing. Partner with CamelBio to streamline your supply chain and accelerate assay development.