YOD1 Rabbit Polyclonal Antibody for WB and ELISA - Q5VVQ6

Core Product Specifications and Parameters

Biological Background: YOD1 Function and Localization

• YOD1 (Ubiquitin thioesterase OTU1, also known as DUBA8, OTUD2) is a member of the OTU family of deubiquitinases.
• It functions as a hydrolase that removes conjugated ubiquitin from proteins, primarily acting on 'Lys-27'-, 'Lys-29'-, and 'Lys-33'-linked polyubiquitin chains, and also hydrolyzes 'Lys-11'-linked chains.
• Key role in endoplasmic reticulum-associated degradation (ERAD): it trims the ubiquitin chain on misfolded lumenal substrates to facilitate their threading through the VCP/p97 pore.
• Localizes to the cytoplasm.
• May participate in macroautophagy, including the clearance of damaged lysosomes. It may recruit PLAA, UBXN6 and VCP to damaged lysosome membranes decorated with K48-linked ubiquitin chains and remove these chains allowing autophagosome formation. Related references: PMID:27753622
• Contains a OTU domain and a zinc-finger domain, classified as a thiol protease requiring metal binding.
• Undergoes alternative splicing, producing multiple isoforms.

Experimental Guidance and Technical Tips

• The polyclonal antibody was raised against a recombinant protein spanning amino acids 1-348 of human YOD1, providing broad epitope coverage.
• For Western blotting, a band at approximately 38 kDa is expected. Include appropriate positive and negative controls (e.g., YOD1 overexpressing lysates and siRNA knockdown samples).
• Cross-reactivity with mouse YOD1 suggests potential for mouse model studies; however, conservation of the immunogen sequence between species should be verified.
• ELISA applications may be optimized using direct or sandwich formats; polyclonal antibodies often serve as robust detection reagents.
• Note that this antibody has been tested for WB and ELISA only; other applications such as IHC or IF are not characterized and would require experimental validation.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research labs with integrated access to validated IVD antibodies, bulk ancillary reagents, and custom sourcing for rare targets. This YOD1 polyclonal antibody supports studies on ubiquitin signaling, ERAD, and autophagy, and extends our portfolio of deubiquitinase-related raw materials. For large-scale or application-specific needs, reach out to explore tailored antibody production and technical consultation.