YES1 Rabbit pAb for WB, IF/ICC, ELISA - P07947

Core Product Specifications and Parameters

Biological Background: YES1 Function and Localization

• YES1 encodes a non-receptor tyrosine kinase of the Src family, functioning as a proto-oncogene (c-Yes) and playing critical roles in cell growth, survival, apoptosis, cell-cell adhesion, cytoskeleton remodeling, and differentiation.
• Upon stimulation by receptor tyrosine kinases (e.g., EGFR, PDGFR, CSF1R, FGFR), YES1 is recruited to phosphorylated receptors and phosphorylates downstream substrates such as PARD3 (promoting epithelial tight junction assembly) and CDK4 (regulating G1 phase cell cycle progression).
• YES1 participates in T-cell migration by phosphorylating DPYSL2 downstream of CXCL12 stimulation, and acts in the CD95L/FASLG signaling pathway to mediate AKT-dependent cell migration.
• It catalyzes phosphorylation of the organic cation transporter OCT2, thereby enhancing its transport activity. Related references: PMID:26979622
• The protein localizes to the cell membrane, cytoplasm, cytoskeleton, centrosome, and cell junctions, reflecting its diverse signaling roles.
• YES1 is expressed in epithelial cells of renal proximal tubules and stomach, keratinocytes of the basal epidermis, and fetal hematopoietic cells in bone marrow and spleen.
• Post-translational modifications include N-terminal myristoylation and palmitoylation for membrane targeting, and autophosphorylation at regulatory tyrosine residues.

Experimental Guidance and Technical Tips

• The immunogen consists of amino acids 1–250 (N-terminal region) of human YES1, suggesting the antibody may detect full-length YES1 (~61 kDa) and possibly N-terminal fragments; users should validate band patterns in their sample types.
• For Western blot (WB), consider using reducing conditions and appropriate loading controls; optimization of antibody dilution is recommended for each experimental system.
• In immunofluorescence (IF) and immunocytochemistry (ICC), YES1 exhibits membranous and cytoplasmic staining; appropriate permeabilization and fixation protocols should be established, and co-localization markers for cell junctions or cytoskeleton may be useful.
• ELISA performance may require checkerboard titration; the antibody’s reactivity with denatured or native protein should be confirmed by the end user.
• Cross-reactivity is reported for human and mouse; when working with other species, verify sequence homology in the immunogen region before use.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive one-stop supply solution for IVD raw materials, delivering validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and tailored sourcing for rare targets to diagnostic manufacturers and research laboratories worldwide. If your work involves tyrosine kinase signaling or cell adhesion targets like YES1, CamelBio can connect you with high-quality reagents and scalable procurement options to support assay development and clinical translation.