XRCC4 Rabbit Polyclonal Antibody for WB - Q13426

Core Product Specifications and Parameters

Biological Background: XRCC4 Function and Localization

• DNA non-homologous end joining (NHEJ) core factor, required for double-strand break repair and V(D)J recombination. Related references: PMID:10757784 PMID:10854421 PMID:12517771
• Acts as a scaffold protein that regulates recruitment of other proteins to DNA double-strand breaks (DSBs). Related references: PMID:15385968 PMID:20852255 PMID:26774286
• Associates with NHEJ1/XLF to form alternating helical filaments that bridge DNA, holding broken ends together. Related references: PMID:21768349 PMID:21775435 PMID:22287571
• The XRCC4-NHEJ1/XLF subcomplex binds to DNA fragments in a highly diffusive manner, keeping ends accessible for further processing. Related references: PMID:27437582
• Key role in NHEJ ligation: interacts with DNA ligase IV (LIG4) to reseal breaks, stabilizing LIG4 and enhancing its joining activity. Related references: PMID:10757784 PMID:10854421 PMID:12517771
• After caspase-3 cleavage, a C‑terminal fragment translocates to the cytoplasm and activates phospholipid scramblase XKR4, promoting phosphatidylserine exposure during apoptosis. Related references: PMID:33725486
• Localizes primarily to the nucleus and chromosomes; cytoplasmic redistribution occurs during apoptosis.
• Widely expressed across human tissues.
• Annotated with keywords: DNA damage, DNA repair, DNA-binding, chromosome, phosphoprotein, disease variant, and alternative splicing.

Experimental Guidance and Technical Tips

• The immunogen (aa 1–336) covers nearly full-length XRCC4; it likely recognizes both full-length protein and the caspase-3 cleaved apoptotic fragment.
• For WB, a band around 38 kDa is expected; a smaller fragment (~30 kDa) may appear in apoptotic samples—validate with positive/negative controls.
• In IHC-P, optimize antigen retrieval and fixation conditions for the tissue of interest; nuclear staining should predominate.
• For IF/ICC, expect nuclear signal under normal conditions; cytoplasmic staining may indicate apoptosis—counterstain with a nuclear marker.
• ELISA can be used for quantitative detection; consider pairing with a capture antibody for sandwich ELISA development.
• Cross-reactivity is reported for human and rat; for other species, verify sequence homology before use.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio empowers your DNA damage and repair research by providing validated antibody pairs, optimized polyclonal and monoclonal antibodies, and bulk ancillary reagents. As your one-stop IVD raw‑material partner, we also specialize in sourcing rare‑target antibodies, supporting your assay development from concept to clinic. Our supply model ensures consistent quality and scalable delivery for diagnostic manufacturers and research laboratories alike.