XPO7 Rabbit pAb for WB and ELISA - Q9UIA9

Core Product Specifications and Parameters

Biological Background: XPO7 Function and Localization

• Exportin-7 (XPO7), also known as Ran-binding protein 16, is a member of the importin-beta superfamily of nuclear transport receptors.
• It mediates the nuclear export of a broad range of protein cargos by forming a cooperative trimeric complex with cargo and Ran-GTP in the nucleus.
• Docking to the nuclear pore complex occurs via interaction with nucleoporins, and cytoplasmic release of cargo is triggered by Ran-GTP hydrolysis induced by RANBP1 and RANGAP1.
• XPO7 shuttles between the nucleus and cytoplasm, with localization observed in both compartments, consistent with its role in nuclear transport.
• Key post-translational modifications include acetylation and phosphorylation, as identified by direct protein sequencing and proteomics.
• High tissue expression is detected in testis, thyroid, and bone marrow, with moderate or low levels in other tissues; it is overexpressed in red blood cells of patients with hereditary non-spherocytic hemolytic anemia.

Experimental Guidance and Technical Tips

• For Western blotting, a predominant band around 124 kDa is expected; optimize blocking and detection steps for your particular sample type and lysis conditions.
• When performing ELISA, consider using the immunogen peptide as a positive control to confirm antibody reactivity and to establish assay conditions.
• Based on the manufacturer's cross-reactivity data (human, mouse, rat), the epitope region likely shares high sequence homology across these species; validate performance in your specific model system.
• Given the large protein size, ensure adequate protein transfer during western blot (e.g., use low-methanol transfer buffer and extend transfer time or current).

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as your integrated IVD raw-material partner, offering validated antibody pairs, optimized monoclonal/polyclonal antibodies, and ancillary reagents for nuclear transport research. Our anti-XPO7 rabbit pAb supports WB and ELISA applications, and we can facilitate bulk orders or custom sourcing of rare-target raw materials to accelerate your diagnostic development workflow from early-stage research to clinical application.