WTAP Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - Q15007

Core Product Specifications and Parameters

Biological Background: WTAP Function and Localization

• WTAP is a regulatory component of the WMM complex that catalyzes N6-methyladenosine (m6A) modification of RNA, influencing mRNA splicing and processing efficiency. Related references: PMID:29507755
• It is essential for the localization of METTL3 and METTL14 to nuclear speckles, where m6A methylation occurs. Related references: PMID:24316715 PMID:24407421 PMID:24981863
• WTAP functions as a splicing regulator, participating in mRNA processing. Related references: PMID:12444081
• It regulates the G2/M cell-cycle transition by stabilizing the CCNA2 mRNA through binding to its 3' UTR. Related references: PMID:17088532
• WTAP interacts with and impairs WT1 DNA-binding activity, thereby inhibiting expression of WT1 target genes. Related references: PMID:17095724
• Subcellular localization includes nuclear speckles, nucleoplasm, and cytoplasm.
• The protein is ubiquitously expressed in tissues and undergoes post-translational modifications such as acetylation and phosphorylation.

Experimental Guidance and Technical Tips

• The immunogen is designed against a recombinant fusion protein spanning amino acids 212–355 of human WTAP (NP_004897.2); this region may be considered for epitope mapping or alternative antibody development.
• For western blot (WB), a band at approximately 44 kDa is expected based on the predicted molecular weight; validate detection in relevant human, mouse, or rat samples given the reported cross-reactivity.
• Immunofluorescence/immunocytochemistry (IF/ICC) can be used to visualize WTAP localization, noting its nuclear speckle, nucleoplasm, and cytoplasmic distribution.
• ELISA applications may be suited for screening or titer determination; appropriate assay conditions should be optimized.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with a comprehensive one-stop platform for IVD raw materials, technical services, and consulting — from concept to clinic. As a trusted sourcing partner, we supply validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare target raw materials to support your WTAP research in RNA modification, splicing biology, and cell cycle regulation. Let CamelBio streamline your procurement and accelerate your assay development.