WNT3A Rabbit pAb for WB, ELISA - P56704

Core Product Specifications and Parameters

Biological Background: Wnt-3a Function and Localization

• Protein Wnt-3a is a secreted signaling protein that acts as a ligand for members of the frizzled family of seven transmembrane receptors.
• Wnt-3a functions in the canonical Wnt signaling pathway, activating transcription factors of the TCF/LEF family.
  Related references: PMID:20093360 PMID:21244856 PMID:24841207
• Wnt-3a mediates self-renewal of stem cells at the bottom of intestinal crypts in vitro.
  Related references: PMID:26902720
• It is required for normal embryonic mesoderm development and formation of caudal somites, as well as normal morphogenesis of the developing neural tube (By similarity).
• Wnt-3a is localized to the extracellular space and extracellular matrix.
• Moderately expressed in placenta, with low levels in adult lung, spleen, and prostate.
• Post-translational modifications include glycosylation, lipidation, and disulfide bond formation, and the protein may undergo alternative splicing.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide spanning amino acids 100-200 of human WNT3A; the antibody may recognize denatured epitopes in western blotting.
• For WB, consider using appropriate loading controls and optimize blocking and antibody dilution for your specific sample type.
• When performing ELISA, validate the coating antigen and antibody concentration in your specific assay system.
• This antibody shows cross-reactivity with human and mouse; for other species, we recommend verifying reactivity experimentally.
• Always validate antibody performance in your own experimental setup, as results can vary with sample preparation and detection methods.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with one-stop access to IVD raw materials, technical services, and consulting from concept to clinic. This WNT3A antibody supports Wnt signaling pathway and stem cell research, and we offer validated antibody pairs, optimized polyclonal and monoclonal antibodies, bulk ancillary reagents, and custom sourcing for rare targets to streamline your assay development.