WISP3 Rabbit Polyclonal Antibody for WB, ELISA - O95389

Core Product Specifications and Parameters

Biological Background: WISP3 Function and Localization

• WISP3 (also known as CCN6, WISP-3) is a member of the CCN family of matricellular proteins, which are secreted glycoproteins involved in cell adhesion, migration, and signaling.
• It plays a crucial role in mitochondrial electron transport and respiration, directly impacting cellular energy metabolism.
  Related references: PMID:27252383
• Through its mitochondrial regulatory functions, WISP3 is essential for normal postnatal skeletal growth and maintaining cartilage homeostasis.
  Related references: PMID:10471507, PMID:27252383
• The protein is predominantly expressed in adult kidney and testis, with weaker expression in placenta, ovary, prostate, small intestine, and skeletal tissues such as synoviocytes and chondrocytes.
• WISP3 localizes to both the secretory pathway and mitochondria, enabling dual roles in extracellular matrix signaling and intracellular energy regulation.
• The gene undergoes alternative splicing, and the mature protein features disulfide bonds and glycosylation, which may influence its stability and functional activity.

Experimental Guidance and Technical Tips

• The immunogen used to generate this antibody is a recombinant fusion protein covering amino acids 42–260 of human WISP3. This region includes key domains, and the antibody is expected to recognize linear epitopes under denaturing conditions (e.g., Western blot).
• For WB applications, a band at approximately 39 kDa is anticipated; however, due to post-translational modifications or alternative splicing, additional bands may appear. Validation with positive controls such as kidney or testis lysates is recommended.
• In ELISA, this polyclonal antibody may serve as a detection reagent; for sandwich ELISA, pairing with a monoclonal capture antibody can be considered to enhance specificity and sensitivity.
• The antibody cross-reacts with mouse and rat WISP3, making it suitable for translational studies across rodent models. Users should verify performance in their specific experimental systems.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio acts as your one-stop sourcing partner for IVD raw materials, offering reliable access to validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare target sourcing. This WISP3 antibody supports research in mitochondrial biology, skeletal development, and related disease areas, and CamelBio can assist in scaling your procurement needs from proof-of-concept to clinical application.