WDR77 Mouse Monoclonal Antibody for WB and ELISA - Q9BQA1

Core Product Specifications and Parameters

Biological Background: WDR77 Function and Localization

• WDR77 (p44/Mep50) is a non-catalytic subunit of the methylosome complex, which includes PRMT5 and CLNS1A, and catalyzes symmetric dimethylation of arginine residues in target proteins (PubMed:11756452). Related references: PMID:11756452

• The methylosome modifies specific Sm proteins, targeting them for assembly into small nuclear ribonucleoprotein (snRNP) core particles; it may also play a role in transcription regulation (PubMed:11756452). Related references: PMID:11756452

• WDR77 methylates Piwi proteins (PIWIL1, PIWIL2, PIWIL4), a modification required for interaction with Tudor domain-containing proteins and localization to meiotic nuage (PubMed:23071334). Related references: PMID:23071334

• Member of the WD repeat protein family; contains multiple WD40 repeats that mediate protein-protein interactions.

• Localizes to both the nucleus and cytoplasm, consistent with its roles in methylation and snRNP assembly.

• Highly expressed in heart, skeletal muscle, spleen, testis, uterus, prostate, and thymus; in testis, found in germ cells and Leydig cells but absent from Sertoli cells.

• Post-translationally modified by phosphorylation; identified as a phosphoprotein in proteomic studies.

• Alternatively spliced isoforms exist; crystal structure is available (3D-structure keyword).

Experimental Guidance and Technical Tips

• The immunogen is a recombinant protein of human WDR77, suggesting the antibody may recognize full-length WDR77; consider validating specificity in relevant human cell lines or tissue lysates.
• For Western blot, the predicted molecular weight is ~37 kDa; however, alternative splicing or phosphorylation may produce additional bands—optimize gel percentage and blocking conditions accordingly.
• For ELISA, pairing with a capture antibody targeting a distinct epitope may improve assay performance; use recombinant WDR77 as a standard for quantification.
• Since WDR77 expression varies across tissues, include appropriate positive and negative controls (e.g., testis vs. negative tissue) in your experiments to confirm target detection.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and researchers with one-stop access to IVD raw materials and technical services. The WDR77 monoclonal antibody supports methylosome and RNA splicing research, and can be complemented by our catalog of validated antibody pairs, bulk reagents, and rare target sourcing capabilities. Partner with CamelBio for streamlined procurement and expert consulting from concept to clinic.