WASF3 Rabbit Polyclonal Antibody for WB and ELISA - Q9UPY6

Core Product Specifications and Parameters

Biological Background: WASF3 Function and Localization

• WASF3, also known as WAVE3, SCAR3, and Brush-1, is an actin-binding protein and a member of the Wiskott-Aldrich syndrome protein (WASP) family. It is encoded by the WASF3 gene in humans (Gene ID: 10810).
• It functions as a downstream effector in the transmission of signals from tyrosine kinase receptors and small GTPases to the actin cytoskeleton, playing a critical role in the regulation of cell morphology and cytoskeletal organization. Related references: PMID:21834987
• WASF3 is localized in the cytoplasm and associates with the cytoskeleton, which is consistent with its role in actin dynamics.
• The protein is expressed in tissues such as ovary and brain, suggesting tissue-specific functions in these organs.
• Key structural features include a coiled coil domain, and the protein undergoes phosphorylation. Alternative splicing generates multiple isoforms, contributing to functional diversity.

Experimental Guidance and Technical Tips

• This polyclonal antibody was raised in rabbit against a synthetic peptide spanning amino acids 200-300 of human WASF3 (isoform NP_001278894.1). The immunogen design targets an internal region, likely accessible in denaturing Western blot conditions.
• The antibody is validated for Western blot (WB) and ELISA applications. For WB, a band corresponding to the predicted molecular weight of approximately 55 kDa may be detected in lysates from human, mouse, or rat samples, given the cross-reactivity data.
• When performing WB, consider using standard SDS-PAGE and transfer protocols, and validate the antibody under your specific experimental conditions. For ELISA, direct or indirect detection may be employed; optimization of coating antigen and antibody dilution is recommended.
• Note that polyclonal antibodies may exhibit lot-to-lot variability; it is advisable to include appropriate positive and negative controls and confirm specificity in your model system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides comprehensive access to IVD raw materials, including validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents. For targets like WASF3 involved in cytoskeletal signaling, our portfolio supports researchers and diagnostic manufacturers in developing reliable assays. Whether you require ready-to-use formulations or custom sourcing for rare targets, CamelBio streamlines the procurement process from concept to clinic, acting as your dedicated sourcing partner.