VSNL1/VILIP-1 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - P62760

Core Product Specifications and Parameters

Biological Background: VSNL1/VILIP-1 Function and Localization

• Visinin-like protein 1 (VILIP-1), encoded by VSNL1, is a calcium-binding protein (UniProt keyword: Calcium) also known as Hippocalcin-like protein 3 (HLP3).
• In vitro, VILIP-1 regulates the inhibition of rhodopsin phosphorylation in a calcium-dependent manner, indicating a role in phototransduction modulation.
• The protein is predominantly expressed in the brain and retina, with neuron-specific localization in both the central and peripheral nervous systems.
• Protein levels of VILIP-1 are elevated in the cerebrospinal fluid of Alzheimer disease patients, suggesting utility as a potential biomarker.
• It belongs to the recoverin family of neuronal calcium sensors and contains EF-hand motifs for calcium binding; it is also myristoylated (keyword: Myristate), facilitating membrane association.
• Classified as a lipoprotein (keyword: Lipoprotein), indicating lipid modification that may influence subcellular targeting.
• The calculated molecular weight is approximately 22.1 kDa (UniProt: 22142 Da), consistent with the observed 22 kDa band.

Experimental Guidance and Technical Tips

• The immunogen spans nearly the full-length human VSNL1 protein (aa 2-191); therefore, the polyclonal antibody is likely to recognize multiple linear epitopes, making it suitable for both denaturing and native applications.
• For Western blot, expect a band at ~22 kDa; use appropriate gel concentrations and loading controls. Validate detection in lysates from brain or retinal tissues where VSNL1 is enriched.
• In immunofluorescence/immunocytochemistry, optimize fixation and permeabilization conditions to preserve the myristoylated membrane-associated protein. Co-staining with neuronal markers (e.g., NeuN, MAP2) is recommended.
• For ELISA, use recombinant VSNL1 or tissue extracts; confirm sensitivity and specificity with appropriate positive and negative controls.
• Since cross-reactivity with mouse and rat is confirmed, the antibody can be used in rodent models of neurological disease; verify sequence homology if extending to other species.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. Our VSNL1/VILIP-1 antibody supports neurology and Alzheimer's disease research, and we can supply validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing. Whatever your IVD development needs, our team is ready to streamline your procurement from discovery to commercialization.