VPS37A Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - Q8NEZ2

Core Product Specifications and Parameters

Biological Background: VPS37A Function and Localization

• VPS37A, also known as Hepatocellular carcinoma-related protein 1 (HCRP1) or ESCRT-I complex subunit VPS37A, is a component of the ESCRT-I complex, a key regulator of vesicular trafficking.
• It is required for the sorting of endocytic ubiquitinated cargos into multivesicular bodies (MVBs), a critical step in the endosomal-lysosomal degradation pathway.
• The protein may play a role in cell growth and differentiation, as suggested by its functional characterization.
• VPS37A localizes to the late endosome membrane and also to the nucleus, indicating potential shuttling or dual functions.
• It is widely expressed in tissues, including heart, brain, placenta, liver, skeletal muscle, kidney, and pancreas, with particularly high abundance in liver. Notably, its expression is strongly decreased or undetected in hepatomas, linking it to hepatocellular carcinoma.
• The gene is associated with hereditary spastic paraplegia (SPG53) and undergoes alternative splicing. The protein is a phosphoprotein and has been identified in proteomics studies.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 224–397 of human VPS37A, a region that may be conserved across species, consistent with the reported cross-reactivity with mouse and rat.
• For Western blot (WB), a band near 44 kDa is expected; use appropriate positive control lysates from human, mouse, or rat tissues.
• In immunofluorescence/immunocytochemistry (IF/ICC), consider permeabilization protocols to access both endosomal membrane and nuclear localizations.
• For ELISA, the antibody can be used as a capture or detection reagent; optimal dilution and pairing should be empirically determined.
• As a polyclonal antibody, lot-to-lot variability should be monitored, and specificity should be confirmed in the relevant experimental system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers and researchers a single point of access to high-quality IVD raw materials, including validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents. This VPS37A polyclonal antibody supports studies in endosomal trafficking, cancer biology, and neurodegenerative disease. For rare target raw-material sourcing or custom development, our team is ready to assist.