VISTA Rabbit pAb - Q9H7M9

Core Product Specifications and Parameters

Biological Background: VISTA Function and Localization

• VISTA (V-type immunoglobulin domain-containing suppressor of T-cell activation) functions as an immunoregulatory receptor that inhibits T-cell responses. Related references: PMID:24691993
• Alternative names include platelet receptor Gi24, stress-induced secreted protein-1 (Sisp-1), and V-set domain-containing immunoregulatory receptor.
• It may promote embryonic stem cell differentiation by inhibiting BMP4 signaling (by similarity).
• VISTA may stimulate MMP14-mediated activation of MMP2. Related references: PMID:20666777
• The protein localizes to the cell membrane as a single-pass transmembrane receptor.
• It is expressed in spleen, on myeloid cells (e.g., CD11b+ monocytes, CD66b+ neutrophils), at low levels on CD4+ and CD8+ T-cells and a subset of NK cells, but not on B cells. Higher expression is seen in placenta, lung, and plasma blood leukocytes.
• VISTA is a glycoprotein and phosphoprotein, containing an immunoglobulin domain and disulfide bonds.

Experimental Guidance and Technical Tips

• For western blotting, consider testing human spleen or lung lysates where VISTA expression is relatively high; validate the band at the expected molecular weight (~34 kDa).
• The immunogen covers the extracellular domain (amino acids 33–194), making the antibody potentially suitable for ELISA detection of soluble or cell-surface VISTA; optimize coating and detection conditions in the relevant sample matrix.
• Due to the polyclonal nature, it may be advisable to include appropriate controls (e.g., lysates from VISTA-overexpressing cells vs. knockdown/knockout) to confirm specificity.
• Cross-reactivity with other species is not indicated; verify reactivity if used with non-human samples.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides a comprehensive one-stop shop for IVD raw materials, supporting diagnostic developers and researchers from early discovery through clinical validation. For immuno-oncology and T-cell regulation studies, we offer optimized antibody pairs, bulk ancillary reagents, and expertise in sourcing rare targets like VISTA. Whether you need validated monoclonal/polyclonal antibodies or custom raw-material procurement, CamelBio bridges the gap between concept and clinic.