VIP Rabbit pAb for WB, IHC-P, ELISA - P01282

Core Product Specifications and Parameters

Biological Background: VIP Function and Localization

• VIP (Vasoactive Intestinal Peptide) is a neuropeptide encoded by the VIP gene, belonging to the glucagon/secretin superfamily. The preproprotein is proteolytically processed to yield multiple bioactive peptides, including VIP and PHM-27 (peptide histidine methionine 27).
• Through binding to its cognate G protein-coupled receptors VPAC1 (VPR1) and VPAC2 (VPR2), VIP mediates pleiotropic effects including vasodilation, smooth muscle relaxation, stimulation of myocardial contractility, and regulation of glycogenolysis. Related references: PMID:1318039, PMID:36385145, PMID:8933357, PMID:15013843, PMID:3654650
• VIP is abundantly expressed in the central and peripheral nervous systems, where it exerts neuroprotective and immunomodulatory functions. Related references: PMID:3456568
• PHM-27 also acts as a potent agonist of the calcitonin receptor CALCR with comparable efficacy to calcitonin. Related references: PMID:15013843
• VIP is a secreted protein primarily localized to the extracellular space, consistent with its role as a circulating hormone.
• Post-translational modifications, including amidation and cleavage on pairs of basic residues, are essential for generating the mature, bioactive peptides.
• Alternative splicing of the VIP gene produces multiple isoforms, potentially expanding functional diversity.
• According to UniProt, the molecular weight of the VIP precursor is approximately 19.2 kDa, aligning with the observed 19 kDa band in Western blot.

Experimental Guidance and Technical Tips

• The immunogen used is a recombinant fragment spanning amino acids 31-170 of human VIP, which covers the mature peptide region. For Western blot, a band around 19 kDa is expected, but additional bands corresponding to precursor or degradation products may also be observed.
• For immunohistochemistry (IHC-P), antigen retrieval methods may be necessary to unmask the epitope in formalin-fixed paraffin-embedded tissues. Validate staining specificity using positive control tissues such as brain, colon, or lung where VIP is known to be expressed.
• In ELISA applications, consider using a competitive or sandwich format with appropriate standards to quantify VIP levels in serum or tissue extracts.
• As a polyclonal antibody, lot-to-lot variability may occur; it is advisable to titrate the antibody in your specific assay conditions for optimal performance.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive range of IVD raw materials and supporting services for diagnostic manufacturers and research laboratories. If you are developing assays targeting neuropeptides like VIP, CamelBio can provide validated antibody pairs, bulk polyclonal and monoclonal antibodies, and ancillary reagents to streamline your workflow from concept to clinic. Our dedicated sourcing team supports rare-target raw material procurement to accelerate your diagnostic development.