Vinculin Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - P18206

Core Product Specifications and Parameters

Biological Background: Vinculin Function and Localization

• Vinculin is an actin filament (F-actin)-binding protein that plays a central role in both cell-matrix adhesion (focal adhesions) and cell-cell adhesion (adherens junctions).
• It potentiates mechanosensing by regulating cell-surface E-cadherin expression and stabilizing the E-cadherin complex, contributing to cell morphology and locomotion.
• The protein localizes to multiple subcellular sites, including focal adhesions, adherens junctions, podosomes, the perinuclear cytoskeleton, and the sarcolemma in muscle cells.
• A muscle-specific splice variant, metavinculin (MV), arises from alternative splicing of the VCL gene and incorporates an additional exon, leading to a larger isoform with unique functions in cardiac and smooth muscle.
• Post-translational modifications such as phosphorylation, acetylation, and palmitoylation regulate vinculin activation, targeting, and protein interactions.
• Mutations in VCL are linked to cardiomyopathies, including dilated cardiomyopathy (CMD1W) and hypertrophic cardiomyopathy (CMH15), underscoring its critical role in cardiac muscle integrity.
• Vinculin serves as a hub for numerous binding partners (e.g., talin, α-actinin, paxillin), integrating mechanical and chemical signals at adhesion sites.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the C-terminal region (amino acids 751–850), which is conserved between vinculin and metavinculin; thus the antibody is expected to recognize both isoforms unless otherwise noted.
• For western blotting, a band near 124 kDa is anticipated; validate specificity using lysates from human, mouse, or rat cells lines known to express vinculin (e.g., HeLa, NIH/3T3, or C2C12).
• In immunofluorescence/immunocytochemistry, optimize fixation (e.g., 4% paraformaldehyde) and permeabilization to preserve focal adhesion structures; co-staining with F-actin or paxillin can serve as positive controls.
• For ELISA, consider a direct or sandwich format; perform titration experiments to determine the optimal coating and antibody concentrations for your specific plate and detection system.
• When working with muscle tissues, be aware that metavinculin migrates at a higher molecular weight and may exhibit differential expression; include appropriate controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with reliable access to IVD raw materials like this validated anti-Vinculin polyclonal antibody, supporting studies in cell adhesion, mechanobiology, and cardiovascular disease. Through a comprehensive portfolio of validated antibody pairs, optimized mono- and polyclonal antibodies, and ancillary reagents, CamelBio streamlines procurement from discovery to clinical application. For rare targets or custom production needs, our team offers agile sourcing and technical support to help accelerate your assay development.