VIMP Rabbit Polyclonal Antibody for WB, ELISA - Q9BQE4

Core Product Specifications and Parameters

Biological Background: Selenoprotein S Function and Localization

• Selenoprotein S (also known as VIMP or SELS) is a selenocysteine-containing protein encoded by the SELENOS gene.
• It plays a critical role in endoplasmic reticulum-associated degradation (ERAD), facilitating the retrotranslocation of misfolded proteins from the ER lumen to the cytosol.
• Functions as a linker between the retrotranslocation channel DERL1 and the cytosolic ATPase VCP/p97, which extracts and ubiquitinates substrates for proteasomal degradation.
• Localizes predominantly to the endoplasmic reticulum membrane and cytoplasm.
• Post-translational modifications include phosphorylation and ubiquitination.
• Contains a single transmembrane helix anchoring it to the ER membrane.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide; consider using denaturing conditions for Western blot to expose linear epitopes.
• Predicted molecular weight is ~21 kDa; ensure adequate separation on SDS-PAGE gels.
• For ELISA, consider using the immunogen peptide as a positive control.
• Validate specificity in relevant cell lines expressing VIMP.
• When testing cross-species reactivity, verify in mouse and rat samples, as sequence homology may affect recognition.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers an integrated sourcing platform for high-quality IVD antibodies and reagents, supporting diagnostic development from concept to clinic. This anti-VIMP antibody enables research into ER stress and protein quality control pathways, critical for understanding cellular homeostasis and disease. Beyond single antibodies, CamelBio can supply validated antibody pairs, bulk ancillary reagents, and assist with rare target raw-material sourcing to streamline your workflow.