Villin1 Rabbit Polyclonal Antibody for WB, ELISA - P09327

Core Product Specifications and Parameters

Biological Background: Villin-1 Function and Localization

• Villin-1 is an epithelial cell-specific actin‑modifying protein that regulates microvillar actin filament reorganization through nucleation, bundling, capping, and severing activities.
• It binds phosphatidylinositol 4,5‑bisphosphate (PIP2) and lysophosphatidic acid (LPA), with LPA binding enhancing SRC‑mediated phosphorylation and inhibiting all actin‑modifying functions, while PIP2 binding inhibits capping and severing but enhances bundling.
• Villin-1 plays a critical role in intestinal epithelial cell morphology, invasion, migration, and apoptosis, and protects against dextran sodium sulfate (DSS)-induced apoptosis by maintaining mitochondrial integrity.
• It enhances hepatocyte growth factor (HGF)-induced epithelial cell motility, chemotaxis, and wound repair, and its actin‑severing activity promotes actin‑based motility of S. flexneri during infection.
• Subcellularly, it localizes to the cytoplasm, cytoskeleton, lamellipodia, ruffles, microvilli, filopodia tips, and filopodia.
• Tissue specificity: Highly expressed in epithelial cells, notably as a major component of microvilli in intestinal epithelial cells, kidney proximal tubule cells, and canalicular microvilli of hepatocytes.
• The protein contains multiple actin‑binding repeats and is regulated by calcium; it undergoes alternative splicing and phosphorylation, as indicated by the keywords: Actin capping, Actin‑binding, Alternative splicing, Apoptosis, Calcium, Cell projection, Cytoplasm, Cytoskeleton, Phosphoprotein.

Experimental Guidance and Technical Tips

• The immunogen spans the full‑length human Villin‑1 (aa 1–827), offering comprehensive epitope coverage; the antibody is suitable for western blot (WB) and ELISA applications.
• For WB, consider testing a range of dilutions (e.g., 1:500–1:2000) and validating in relevant epithelial cell lines (e.g., Caco‑2, HeLa) or tissue lysates from human, mouse, or rat.
• Optimization of blocking and detection conditions is recommended; the predicted molecular weight is ~93 kDa, but post‑translational modifications may cause shifts.
• In ELISA, pair with a suitable secondary antibody and validate using recombinant or native Villin‑1 as standard.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one‑stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. For Villin‑1 and epithelial cell research, we supply validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and can source rare‑target raw materials upon request. Let CamelBio be your reliable supply‑chain partner for high‑quality research reagents and tailored procurement solutions.