VEPH1 Rabbit Polyclonal Antibody for WB and ELISA - Q14D04

Core Product Specifications and Parameters

Biological Background: VEPH1 Function and Localization

• VEPH1 (ventricular zone-expressed PH domain-containing protein homolog 1), also referred to as Protein melted, is encoded by the VEPH1 gene (KIAA1692).
• It functions as an inhibitor of TGF-beta signaling by binding to TGFBR1 and preventing the release of phosphorylated SMAD2, thereby blocking its nuclear translocation and downstream transcriptional activity.
• Beyond TGF-beta, VEPH1 may modulate FOXO, Hippo, and Wnt signaling pathways, suggesting a broader role in cellular signaling networks.
• The protein localizes to the cell membrane, consistent with its membrane-associated regulatory roles.
• Alternative splicing of VEPH1 generates multiple isoforms, adding to its functional diversity.
• According to UniProt annotations, VEPH1 is a reference proteome member and has been identified in mass spectrometry-based proteomics studies.

Experimental Guidance and Technical Tips

• This antibody was raised against a recombinant fragment corresponding to amino acids 448-547, which lies within a central region of the protein. It may detect multiple isoforms due to alternative splicing.
• For Western blot, the predicted molecular weight is 95 kDa, but bands may shift due to post-translational modifications. Validate in the relevant cell or tissue lysates.
• For ELISA, consider direct or sandwich formats, and optimize coating antigen concentration. Cross-reactivity with mouse allows for studies in mouse models.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for diagnostic manufacturers, laboratories, and research institutes, providing one-stop access to IVD raw materials, technical services, and consulting from concept to clinic. This VEPH1 polyclonal antibody is part of our extensive portfolio for cellular signaling and cancer research, and we also offer validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and specialized sourcing for rare targets.