VEGFC Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - P49767

Core Product Specifications and Parameters

Biological Background: VEGFC Function and Localization

• VEGFC, also known as Vascular endothelial growth factor C, Flt4 ligand (Flt4-L), or vascular endothelial growth factor-related protein (VRP), is a secreted growth factor encoded by the VEGFC gene.
• It plays a critical role in angiogenesis and lymphangiogenesis, stimulating endothelial cell proliferation, migration, and survival, as well as regulating vascular permeability.
• VEGFC exerts its functions by binding and activating the receptor tyrosine kinases KDR/VEGFR2 and FLT4/VEGFR3.
• It is essential for the development of the venous and lymphatic vascular systems during embryogenesis and maintains differentiated lymphatic endothelium in adults.
• VEGFC is a secreted protein, localized extracellularly after processing and signal peptide cleavage.
• Post-translational modifications include disulfide bond formation and glycosylation, and the protein undergoes cleavage at pair of basic residues to generate the mature active form.
• Tissue expression is broad: high expression in spleen, lymph node, thymus, appendix, bone marrow; also detected in heart, placenta, skeletal muscle, ovary, small intestine, prostate, testis, and colon.
  Related references:
  PMID:8700872 PMID:9247316 PMID:8617204

Experimental Guidance and Technical Tips

• Immunogen design: The antibody was raised against a synthetic peptide corresponding to amino acids 200-300 of human VEGFC (NP_005420.1). This region lies within the central domain of the precursor protein and may be conserved across species, contributing to the observed cross-reactivity with mouse.
• For Western blotting (WB), the predicted molecular weight of VEGFC is approximately 47 kDa (unprocessed precursor) but may appear as multiple bands due to proteolytic processing and glycosylation; consider using appropriate reducing conditions and validate in relevant cell lysates or conditioned media.
• For immunohistochemistry (IHC-P), antigen retrieval and antibody dilution should be optimized in the specific tissue type; positive control tissues include spleen, lymph node, or placenta.
• ELISA applications may require pairing with a suitable detection antibody; confirm reactivity in the intended sample matrix.
• Always validate the antibody in your experimental system, as performance may vary with sample preparation and conditions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a dedicated one-stop sourcing partner for IVD raw materials, providing validated antibodies, bulk ancillary reagents, and tailored procurement for rare targets. With this anti-VEGFC polyclonal antibody, researchers investigating angiogenesis, lymphangiogenesis, and vascular biology can reliably detect human and mouse VEGFC. As your sourcing bridge, CamelBio connects you to optimized monoclonal/polyclonal antibodies, antibody pairs, and a full range of supportive raw materials—helping streamline your diagnostic or research workflows from concept to clinic.