VCAM1 Rabbit Monoclonal Antibody for WB, IHC-P, ELISA - P19320

Core Product Specifications and Parameters

Biological Background: VCAM1 Function and Localization

• VCAM1 (Vascular cell adhesion protein 1) is a cell adhesion glycoprotein predominantly expressed on the surface of endothelial cells, critical for immune surveillance and inflammation. Related references: PMID:31310649
• It acts as a major regulator of leukocyte adhesion to the endothelium via integrin interactions. Related references: PMID:10209034
• During inflammatory responses, VCAM1 engagement activates calcium channels and RAC1, promoting leukocyte transendothelial migration. Related references: PMID:22970700
• Serves as a quality-control checkpoint in bone marrow by delivering a 'don't-eat-me' signal through MHC class-I presentation. Related references: PMID:35210567
• Multiple alternatively spliced isoforms yield proteins with apparent molecular weights of 71, 74, and 81 kDa; the canonical form is 81 kDa.
• Subcellular localization includes the cell membrane and secretion; it is expressed on inflamed vascular endothelium and on macrophage-like and dendritic cells.
• Contains immunoglobulin-like domains, disulfide bonds, N-glycosylation sites, and ubiquitination modifications.

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 25-698 of the extracellular domain, suggesting the antibody may detect multiple VCAM1 isoforms.
• For western blot, expect bands around 71–81 kDa; optimize reducing conditions and load sufficient protein from endothelial or inflammatory samples.
• In immunohistochemistry (paraffin), antigen retrieval may be required; validate in tissues with known VCAM1 expression, such as inflamed endothelium.
• For ELISA, the antibody can be tested as a capture or detection reagent against recombinant VCAM1 or cell lysates.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides comprehensive one-stop access to IVD raw materials, technical services, and consulting for diagnostic development. This VCAM1 monoclonal antibody supports research in inflammation and immune cell trafficking, and CamelBio can supply validated antibody pairs, bulk ancillary reagents, and custom sourcing for rare target materials to streamline your assay development from concept to clinic.