VARS Rabbit pAb for WB, IHC-P, IF/ICC, ELISA - P26640

Core Product Specifications and Parameters

Biological Background: VARS Function and Localization

• Encodes valine--tRNA ligase (ValRS), also referred to as G7a or VARS2, a member of the class-I aminoacyl-tRNA synthetase family.
• Catalyzes the ATP-dependent attachment of valine to its cognate tRNA(Val) during protein biosynthesis.
• Exhibits post-translational modifications including acetylation and phosphorylation, which may regulate its enzymatic activity.
• The full-length protein has a molecular weight of approximately 140 kDa.
• UniProt keywords associate VARS with neurological disease variants, including epilepsy and intellectual disability.
• Alternative splicing generates multiple transcript isoforms, potentially modulating tissue-specific functions.
• Contains conserved ATP-binding and nucleotide-binding domains essential for ligase activity.

Experimental Guidance and Technical Tips

• The immunogen (amino acids 1-280) targets the N-terminal region of human VARS; this epitope selection may provide specificity across splice variants.
• For Western blot, a band around 140 kDa is anticipated; consider using a high-molecular-weight marker in gel electrophoresis.
• In IHC-P and IF/ICC, validate antibody reactivity in appropriate tissue or cell controls alongside recommended antigen retrieval methods.
• Cross-reactivity with mouse and rat orthologs is indicated; however, verify performance in the specific experimental context before large-scale studies.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio empowers diagnostic developers and research laboratories with streamlined access to IVD raw materials, including validated antibody pairs, monoclonal and polyclonal antibodies, and bulk ancillary reagents. This VARS rabbit polyclonal antibody, targeting valyl-tRNA synthetase, supports investigations into protein translation mechanisms and related neurological disorders. Through its integrated sourcing and technical consulting, CamelBio bridges the gap from early-stage concept to clinical application.