VANGL2 Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - Q9ULK5

Core Product Specifications and Parameters

Biological Background: VANGL2 Function and Localization

• VANGL2, also known as Vang-like protein 2, Loop-tail protein 1 homolog, Strabismus 1, and Van Gogh-like protein 2, is a multi-pass transmembrane protein involved in planar cell polarity and developmental patterning.
• It plays a critical role in the regulation of stereociliary bundle orientation in the cochlea, essential for hearing (By similarity).
• VANGL2 is required for early morphogenesis, contributing to the development of axial midline structures and the neural plate (By similarity).
• It facilitates polarization and movement of myocardializing cells in the cardiac outflow tract via RHOA signaling (By similarity).
• The protein is necessary for the cell surface localization of Frizzled receptors FZD3 and FZD6 in the inner ear (By similarity).
• Subcellularly, VANGL2 localizes to the cell membrane and contains several transmembrane helices.
• Mutations in VANGL2 are associated with disease variants; the protein is a key component of the non-canonical Wnt/planar cell polarity pathway.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (aa 1-110), which may enable detection of full-length VANGL2 and potential isoforms. Validate specificity in relevant sample systems.
• For Western blot (WB), a prominent band near 60 kDa is expected; use reducing conditions and appropriate loading controls.
• For immunohistochemistry on paraffin sections (IHC-P), antigen retrieval and blocking conditions should be empirically optimized to minimize background.
• For ELISA, consider pairing with a capture antibody or using as a detection antibody; polyclonal antibodies may provide high sensitivity but require rigorous specificity controls.
• Cross-reactivity with mouse VANGL2 is reported; verify performance in mouse models if applicable.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD raw materials, offering validated antibody pairs, recombinant proteins, and ancillary reagents. This VANGL2 polyclonal antibody supports research in planar cell polarity and developmental biology. Beyond this catalog product, CamelBio can source custom monoclonal antibodies, bulk quantities, and matched antigen controls to streamline your assay development for diagnostic applications.