VAMP8 Rabbit mAb for WB, IHC-P, ELISA - Q9BV40

Core Product Specifications and Parameters

Biological Background: VAMP8 Function and Localization

• VAMP8 (Vesicle-associated membrane protein 8) is a v-SNARE protein essential for intracellular membrane fusion, mediating the docking and fusion of vesicles with target membranes.
• It forms a SNARE complex with STX11 and SNAP23, critical for regulated exocytosis of cytotoxic granules in NK cells and cytotoxic T-lymphocytes. Related references: PMID:28265073
• VAMP8 plays a key role in autophagy by forming a complex with SNAP29 and STX17, facilitating the fusion of autophagosomes with lysosomes. Related references: PMID:23217709, PMID:25686604
• Required for dense-granule secretion in platelets, supporting hemostasis and thrombosis. Related references: PMID:12130530
• Participates in the activation of the type I interferon antiviral response through a TRIM6-dependent mechanism. Related references: PMID:31694946
• Localizes to lysosomes, early and late endosomes, the cell membrane, and zymogen granule membranes, reflecting its diverse roles in secretion and organelle trafficking.
• Highly expressed in platelets, where it mediates dense-granule release, and subject to post-translational modifications including acetylation and phosphorylation.

Experimental Guidance and Technical Tips

• For Western blotting, use denaturing and reducing conditions; VAMP8 migrates at ~11 kDa. Consider prolonged transfer times for small proteins.
• In immunohistochemistry (IHC-P), antigen retrieval and permeabilization steps are critical for detecting this membrane-bound protein. Optimize antibody dilution for the specific tissue type.
• This antibody is validated for human, mouse, and rat; species reactivity should be confirmed in the experimental model if cross-reactivity data is limited.
• For ELISA applications, pair with a suitable capture/detection antibody targeting a non-overlapping epitope, as the immunogen lies within the N-terminal region.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your dedicated partner for IVD raw-material sourcing, offering a comprehensive portfolio from optimized monoclonal antibodies to rare target reagents. The VAMP8 Rabbit mAb supports research into membrane trafficking, exocytosis, and autophagy pathways, critical for diagnostic and therapeutic development. Whether you need validated antibody pairs, bulk ancillary reagents, or assistance sourcing antibodies against challenging targets, CamelBio streamlines your supply chain from discovery to commercial application.