VAMP2 Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - P63027

Core Product Specifications and Parameters

Biological Background: VAMP2 Function and Localization

• VAMP2 (synaptobrevin-2) is a vesicle-associated membrane protein acting as a v-SNARE, crucial for intracellular membrane fusion events.
• It assembles with the t-SNAREs SNAP25 and STX1A to form a trans-SNARE complex that drives the fusion of synaptic vesicles with the presynaptic plasma membrane, enabling neurotransmitter release. Related references: PMID:10036234, PMID:30929742
• Additionally, VAMP2 plays a role in compensatory endocytosis for synaptic vesicle recycling.
• In adipocytes, it is involved in the insulin-stimulated exocytosis of GLUT4 storage vesicles, contributing to glucose homeostasis.
• Independent of its SNARE activity, VAMP2 may modulate the gating properties of the voltage-dependent potassium channel KCNB1.
• The protein is localized primarily to the membranes of synaptic vesicles and the cell membrane.
• Expression is enriched in the nervous system and skeletal muscle.
• Mutations in VAMP2 have been associated with intellectual disability, highlighting its critical role in neuronal function.

Experimental Guidance and Technical Tips

• For Western blot (WB), whole cell lysates from neuronal cell lines (e.g., SH-SY5Y) or brain tissue are recommended; expect a band at approximately 13 kDa. Consider using a blocking buffer optimized for low-molecular-weight proteins.
• For immunohistochemistry (IHC-P), validation in formalin-fixed, paraffin-embedded brain sections is suggested. Antigen retrieval may be necessary.
• For ELISA, this polyclonal antibody can be used as a capture or detection reagent, but optimal dilutions should be determined empirically.
• The immunogen corresponds to an N-terminal peptide (residues 1-100), so the antibody is likely to recognize both full-length and truncated forms; confirm specificity in your experimental system.
• Given the cross-reactivity with mouse and rat, this antibody is suitable for studies in common rodent models, but validate cross-reactivity in your specific application.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a one-stop sourcing partner for IVD manufacturers, offering a comprehensive range of raw materials including validated monoclonal/polyclonal antibodies, bulk ancillary reagents, and procurement services for rare targets. Leveraging a global supply network, we streamline the sourcing of high-performance antibodies like this anti-VAMP2 polyclonal antibody, supporting diagnostic and research applications in neuroscience and synaptic function. Contact us for custom antibody development or bulk orders tailored to your IVD production needs.