β-Synuclein Rabbit pAb for WB, ELISA - Q16143

Core Product Specifications and Parameters

Biological Background: β-Synuclein Function and Localization

• β-Synuclein is encoded by the SNCB gene and belongs to the synuclein family, which also includes α-synuclein (SNCA) and γ-synuclein (SNCG).
• It is a non-amyloid component of senile plaques found in Alzheimer disease and may act as a regulator of α-synuclein aggregation, potentially mitigating its toxic effects.
• β-Synuclein protects neurons from staurosporine and 6-hydroxydopamine (6-OHDA)-induced caspase activation in a p53/TP53-dependent manner and contributes to restoring α-synuclein's anti-apoptotic function after 6-OHDA insult.
• Unlike α-synuclein, β-synuclein is not found in the Lewy bodies associated with Parkinson disease.
• Subcellularly, β-synuclein localizes to the cytoplasm.
• It is predominantly expressed in the brain, with enrichment at presynaptic nerve terminals.
• Keywords include phosphorylation, indicating post-translational modification may regulate its function.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 1-134 of human β-synuclein, covering the N-terminal region; consider this when designing experiments that may involve N-terminal processing or interactions.
• For Western blotting, verify the expected band at approximately 14 kDa using appropriate positive controls, such as human, mouse, or rat brain lysates, given the reported cross-reactivity.
• For ELISA, consider pairing with a validated detection antibody or using the antibody as a capture reagent; titrate both for optimal signal-to-noise ratio.
• Perform standard validation steps, such as preincubation with the immunogen to confirm specificity, especially when analyzing tissues with high synuclein family expression.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive IVD raw-material partner, offering one-stop access to validated antibody pairs, optimized polyclonal antibodies, and bulk ancillary reagents for neurodegenerative disease research. Whether you need high-quality anti-β-synuclein antibodies for immunoassay development or seek rare target raw-material sourcing for synucleinopathy studies, our growing portfolio and technical consultation support your diagnostic pipeline from concept to clinic.