UTY Rabbit Polyclonal Antibody for WB, ELISA - O14607

Core Product Specifications and Parameters

Biological Background: UTY Function and Localization

• UTY is a male-specific histone demethylase that catalyzes removal of trimethylation from lysine 27 on histone H3 (H3K27me3), a key epigenetic mark associated with gene repression.
• Encoded by the UTY gene (also known as KDM6C) on the Y chromosome, its expression is restricted to males, contributing to sex-specific chromatin regulation.
• The protein displays relatively low demethylase activity in vitro compared to its X-linked homolog UTX (KDM6A).
• UTY is primarily localized to the nucleus, consistent with its function as a chromatin-modifying enzyme.
• It belongs to the Jumonji C (JmjC) domain-containing family of histone demethylases, requiring iron and zinc cofactors for catalytic activity.
• Alternative splicing produces multiple isoforms; the canonical sequence has a molecular weight of approximately 150 kDa.
• In addition to its demethylase function, UTY may participate in protein scaffolding through its tetratricopeptide repeat (TPR) domains.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (amino acids 1-70) of human UTY, which may facilitate detection of the full-length protein and certain isoforms.
• For Western blotting, include appropriate nuclear loading controls and use a gel percentage suitable for resolving a 150 kDa protein (e.g., 8% SDS-PAGE) with optimized transfer conditions.
• Due to cross-reactivity with mouse and rat, consider validating species-specific reactivity in your experimental model; the antibody may also recognize conserved epitopes in other mammals.
• In ELISA applications, confirm binding specificity using the immunogen peptide or recombinant protein as a positive control.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and research institutes by providing reliable access to IVD raw materials like this UTY polyclonal antibody, tailored for epigenetics and Y chromosome research. We offer validated antibody pairs, optimized polyclonal and monoclonal reagents, bulk ancillary supplies, and sourcing for rare targets — helping you bridge the gap from concept to clinic efficiently.