USP9X Rabbit Monoclonal Antibody for WB, IHC-P, IF/ICC, ELISA - Q93008

Core Product Specifications and Parameters

Biological Background: USP9X Function and Localization

• USP9X (Ubiquitin carboxyl-terminal hydrolase 9X), also known as FAF, FAM, hFAM, DFFRX, MRX99, is a large deubiquitinase enzyme encoded by the USP9X gene located on the X chromosome. It belongs to the peptidase C19 family of ubiquitin-specific proteases.
• Acting as a thiol protease, USP9X specifically hydrolyzes polyubiquitin chains with a preference for Lys-11 linkages, followed by Lys-63, Lys-48, and Lys-6 linkages, thereby removing ubiquitin from target proteins and regulating protein turnover. Related references: PMID:30914461
• USP9X is an essential component of the TGF-beta/BMP signaling cascade, where it deubiquitinates monoubiquitinated SMAD4, opposing the E3 ubiquitin-protein ligase TRIM33 and promoting signal transduction. Related references: PMID:19135894
• In mitosis, USP9X regulates chromosome alignment and segregation by controlling the localization of BIRC5/survivin to mitotic centromeres, thus ensuring proper cell division. Related references: PMID:16322459
• USP9X deubiquitinates and stabilizes multiple proteins involved in DNA repair (ALKBH3), RNA demethylation (ALKBH5), and mTORC2 complex assembly (RICTOR), highlighting its role in maintaining cellular homeostasis. Related references: PMID:25944111 PMID:37454738 PMID:33378666
• The enzyme also participates in peroxisome import by deubiquitinating PEX5, resets the circadian clock via BMAL1 stabilization, and inhibits the Hippo signaling pathway through AMOTL2 deubiquitination. Related references: PMID:22371489 PMID:29626158 PMID:26598551 PMID:34404733
• Subcellularly, USP9X localizes to the cytosol, growth cones, and the cilium axoneme, consistent with its roles in neuronal development and cell migration.
• Widely expressed in embryonic and adult tissues, mutations in USP9X are associated with X-linked intellectual disability (MRX99, XLID99), underscoring its importance in neurodevelopment.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (aa 1-100) of human USP9X; the antibody may recognize full-length USP9X as well as any N-terminal fragments. Consider this when interpreting band patterns in Western blot.
• Due to the high molecular weight of USP9X (~290 kDa), use low-percentage SDS-PAGE gels (e.g., 6%) and extended wet transfer times (e.g., overnight at 4°C) for optimal resolution and transfer in WB.
• For IHC-P and IF/ICC, standard antigen retrieval techniques (heat-mediated citrate or EDTA buffer) are recommended, but fixation conditions should be validated for the specific sample type.
• In ELISA, the synthetic peptide immunogen can serve as a positive control; however, cross-reactivity with mouse and rat USP9X should be verified if using rodent samples.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing bridge for IVD raw materials, covering every stage from concept to clinic. For researchers and manufacturers focusing on deubiquitinases and protein degradation pathways, we offer validated antibody pairs, bulk monoclonal antibodies like this USP9X Rabbit mAb, and ancillary reagents to streamline your assay development and production needs.