USP7/HAUSP Rabbit pAb - Q93009

Core Product Specifications and Parameters

Biological Background: USP7/HAUSP Function and Localization

• USP7, also known as HAUSP, is a deubiquitinating enzyme that removes ubiquitin from target proteins, regulating their stability and activity.
• It plays a dual role in the p53-MDM2 pathway, deubiquitinating both p53 and MDM2 to modulate p53-dependent transcription and apoptosis (PubMed:11923872, PubMed:15053880, PubMed:26786098). Related references: PMID:11923872 PMID:15053880 PMID:26786098
• Participates in transcription-coupled nucleotide excision repair by deubiquitinating ERCC6 at UV damage sites (PubMed:22466611, PubMed:22466612). Related references: PMID:22466611 PMID:22466612
• Maintains DNA methylation through deubiquitination of UHRF1 and DNMT1, preventing their degradation (PubMed:21745816, PubMed:22411829). Related references: PMID:21745816 PMID:22411829
• Regulates circadian rhythms by stabilizing CRY1 and CRY2 deubiquitination (PubMed:27123980). Related references: PMID:27123980
• Localizes to the nucleus, cytoplasm, PML bodies, and chromosomes, enabling interaction with diverse substrates.
• Overexpressed in prostate cancer and widely expressed in tissues, with detected expression in neural progenitor cells.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a large central region (amino acids 206-1102), which may detect full-length USP7 and potentially some isoforms. Confirm specificity by Western blot using appropriate lysates (human, mouse, rat) and consider siRNA knockdown controls.
• For Western blot, a band around 128 kDa is expected. Use reducing conditions and standard transfer protocols; note that USP7 may undergo post-translational modifications that slightly alter migration.
• For immunofluorescence (IF/ICC) and immunohistochemistry (IHC-P), optimize fixation and permeabilization conditions. Antigen retrieval may be required for IHC-P. Validate with positive and negative tissue/cell controls.
• Since this is a polyclonal antibody, lot-to-lot variation may occur; consider validating each new lot for consistency in target applications.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive range of IVD raw materials, including validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents. For USP7 research in protein ubiquitination and degradation pathways, we provide reliable access to this USP7/HAUSP rabbit polyclonal antibody, ideal for developing diagnostic assays and exploring protein stability mechanisms. As your sourcing bridge, CamelBio supports every stage from discovery to clinical application with tailored technical consulting.