USP7/HAUSP Rabbit Monoclonal Antibody for WB, IHC-P, ELISA - Q93009

Core Product Specifications and Parameters

Biological Background: USP7/HAUSP Function and Localization

• USP7/HAUSP is a deubiquitinating enzyme that removes ubiquitin moieties from target proteins, regulating their stability and function. It preferentially cleaves 'Lys-48'-linked ubiquitin chains (PubMed:22689415).
  Related references:
  PMID:22689415

• USP7 plays a dual role in the p53 pathway: it deubiquitinates and stabilizes p53, enhancing p53-mediated transcription and apoptosis (PubMed:11923872, PubMed:25283148, PubMed:26786098), and also deubiquitinates MDM2, preventing its self-ubiquitination and promoting MDM2-mediated p53 degradation (PubMed:15053880, PubMed:16845383, PubMed:18566590).
  Related references:
  PMID:11923872 | PMID:25283148 | PMID:26786098 | PMID:15053880 | PMID:16845383 | PMID:18566590

• It deubiquitinates FOXO4 in a hydrogen peroxide-dependent manner, inhibiting FOXO4 transcriptional activity (PubMed:16964248), and regulates PTEN relocation from the nucleus to the cytoplasm via interaction with DAXX and PML (PubMed:18716620).
  Related references:
  PMID:16964248 | PMID:18716620

• USP7 is involved in DNA damage repair: it promotes TC-NER by deubiquitinating and stabilizing ERCC6 at UV-damaged sites (PubMed:22466611, PubMed:22466612) and maintains DNA methylation by deubiquitinating DNMT1 and UHRF1 (PubMed:21745816, PubMed:22411829).
  Related references:
  PMID:22466611 | PMID:22466612 | PMID:21745816 | PMID:22411829

• It regulates chromatin structure by deubiquitinating histone H2B and components of the Polycomb PRC1-like complex (PubMed:20601937), and modulates circadian rhythm by stabilizing CRY1 and CRY2 (PubMed:27123980).
  Related references:
  PMID:20601937 | PMID:27123980

• USP7 contributes to viral pathogenesis: it stabilizes herpesvirus ICP0 and EBV EBNA1, facilitating PML degradation and viral replication.

• Tissue expression: USP7 is widely expressed, with overexpression noted in prostate cancer; it is present in neural progenitor cells (PubMed:21258371).
  Related references:
  PMID:21258371

• Subcellular localization: USP7 dynamically shuttles between the nucleus, cytoplasm, and PML bodies, and associates with chromosomes during mitosis.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 100–200 within the N-terminal domain of human USP7. This region is conserved across human, mouse, and rat, supporting cross-reactivity.
• For Western blot, expect a band around 128 kDa. Use a prestained protein ladder and include appropriate positive and negative controls.
• For IHC-P, optimize antigen retrieval conditions and primary antibody dilution using known USP7‑expressing tissues (e.g., prostate cancer). Validate staining specificity with isotype controls.
• ELISA performance can be assessed using recombinant USP7 protein; consider a checkerboard titration to determine optimal antibody concentration.
• Cross-reactivity with mouse and rat USP7 has been reported. When using rodent models, confirm reactivity in the relevant sample system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with a one-stop sourcing solution for IVD raw materials. Our USP7/HAUSP antibody portfolio offers validated reagents suitable for deubiquitinase research and assay development. Beyond antibodies, we supply bulk ancillary reagents, recombinant proteins, and technical consulting to accelerate your projects from concept to clinical application.