USP39 Rabbit Monoclonal Antibody for WB, IHC-P, IF/ICC, ELISA - Q53GS9

Core Product Specifications and Parameters

Biological Background: USP39 Function and Localization

• USP39 is a deubiquitinating enzyme and an integral component of the U4/U6.U5 tri-snRNP complex, essential for pre-mRNA splicing.
  Related references: PMID:11350945 PMID:26912367
• It facilitates DNA double-strand break repair by mediating the recruitment of XRCC4/LIG4 for non-homologous end-joining.
  Related references: PMID:34614178
• USP39 stabilizes CHK2 via deubiquitination, thereby regulating apoptosis and the G2/M cell cycle checkpoint following DNA damage.
  Related references: PMID:30771428
• The protein contributes to antiviral innate immunity by stabilizing STAT1 and modulating type I interferon signaling.
  Related references: PMID:33127822
• USP39 acts as a negative regulator of NF-κB activation through 'Lys-48'-linked deubiquitination of NFKBIA.
  Related references: PMID:36651806
• It is involved in cytokinesis and the spindle checkpoint by regulating AURKB mRNA levels.
  Related references: PMID:18728397
• USP39 is required for B-cell development by regulating immunoglobulin gene rearrangement in a spliceosome-dependent manner.
• The protein localizes to the nucleus and has a calculated molecular weight of approximately 65 kDa (UniProt: 65,381 Da).

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide corresponding to amino acids 466–565 of human USP39, suggesting specificity for the C-terminal region. Confirm sequence alignment for cross-reactivity in your species.
• For Western blot (WB), a dominant band near 65 kDa is expected; include appropriate positive and negative controls.
• In immunohistochemistry (IHC-P) and immunofluorescence (IF/ICC), anticipate nuclear staining consistent with USP39’s localization. Optimize antigen retrieval and antibody dilution empirically.
• The antibody is predicted to cross-react with human, mouse, and rat USP39; validate in your model system.
• For ELISA, use standard direct or sandwich protocols; confirm coating antigen and detection conditions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD manufacturers and research laboratories, offering validated monoclonal antibodies like this anti-USP39 clone along with ancillary reagents and bulk supply options. Our portfolio covers spliceosome-related proteins, deubiquitinases, and DNA damage response targets, with flexible procurement from single vials to production-scale quantities. Whether you need matched antibody pairs, recombinant proteins, or custom raw-material sourcing, CamelBio accelerates your diagnostic development from concept to clinic.