USP10 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - Q14694

Core Product Specifications and Parameters

Biological Background: USP10 Function and Localization

• USP10 (Ubiquitin carboxyl-terminal hydrolase 10) is a thiol protease that functions as a deubiquitinating enzyme, removing ubiquitin from various substrates to regulate protein stability, signaling, and trafficking. Related references: PMID:11439350 PMID:18632802 PMID:31981475

• In unstressed cells, USP10 localizes to the cytoplasm where it specifically deubiquitinates and stabilizes p53/TP53 by counteracting MDM2-mediated degradation. Upon DNA damage, USP10 translocates to the nucleus to further deubiquitinate p53 and facilitate the DNA damage response. Related references: PMID:20096447

• USP10 controls autophagy by deubiquitinating BECN1, a key autophagy regulator, thereby stabilizing PIK3C3/VPS34 complexes. This creates a regulatory loop where PIK3C3/VPS34 complexes, in turn, regulate USP10 stability, impacting p53 levels. Related references: PMID:21962518

• USP10 plays a critical role in ribosome quality control and stress granule dynamics. It inhibits stress granule assembly by lowering G3BP1/2 valency and mediates deubiquitination of 40S ribosomal proteins (RPS2, RPS3, RPS10) to promote subunit recycling after translation stalling. Related references: PMID:27022092 PMID:32302570 PMID:31981475 PMID:34348161 PMID:34469731

• USP10 deubiquitinates CFTR in early endosomes, enhancing its endocytic recycling and cell surface expression. Related references: PMID:19398555

• USP10 acts as a negative regulator of NF-κB signaling by deubiquitinating IKBKG or TRAF6 in response to IL-1β or DNA damage, and also blocks RIG-I-mediated MAVS activation during RNA virus infection by removing unanchored K63-linked polyubiquitin from MAVS. Related references: PMID:25861989 PMID:37582970

• USP10 is widely expressed and localizes to the cytoplasm, nucleus, and early endosomes. Post-translational modifications include acetylation and phosphorylation, and alternative splicing produces multiple isoforms.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the C-terminal region (aa 499–798) of human USP10. This region may recognize multiple isoforms arising from alternative splicing.
• For Western blotting, the predicted molecular weight is ~87 kDa; verify band specificity with appropriate controls, especially in samples with active deubiquitinase processes.
• For immunofluorescence (IF/ICC), note that USP10 shuttles between cytoplasm and nucleus. Consider using cell fractionation or carefully optimized fixation/permeabilization protocols to capture both localizations.
• The antibody is validated for WB, IF/ICC, and ELISA. Optimize working dilutions empirically for each application and sample type.
• Because USP10 is involved in stress responses (e.g., DNA damage, ribosome stalling), include appropriate positive and negative controls in your experiments.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides a comprehensive portfolio of IVD raw materials, including validated antibody pairs, monoclonal and polyclonal antibodies, and ancillary reagents, supporting diagnostic developers and research labs from concept to clinic. This USP10 antibody is well-suited for studies in cancer biology, autophagy, protein quality control, and innate immunity. For bulk sourcing or custom requirements, our team is ready to streamline your procurement and accelerate your project timeline.