USP10 Rabbit Monoclonal Antibody for WB, IF/ICC, ELISA - Q14694

Core Product Specifications and Parameters

Biological Background: USP10 Function and Localization

• USP10, also known as ubiquitin carboxyl-terminal hydrolase 10, deubiquitinating enzyme 10, or ubiquitin thioesterase 10, is a thiol protease belonging to the ubiquitin-specific processing protease family.
• It functions as a hydrolase that specifically removes conjugated ubiquitin from target proteins, playing a critical role in regulating protein stability and signaling.
• USP10 is a key regulator of p53/TP53: it deubiquitinates and stabilizes p53 in the cytoplasm under unstressed conditions, and translocates to the nucleus upon DNA damage to further regulate p53-dependent DNA damage responses. Related references: PMID:20096447
• It also deubiquitinates BECN1, a key autophagy regulator, thereby stabilizing PIK3C3/VPS34-containing complexes and contributing to a regulatory loop that controls autophagy and p53 levels. Related references: PMID:21962518
• USP10 participates in ribosome quality control by deubiquitinating 40S ribosomal subunit proteins (RPS2/us5, RPS3/us3, RPS10/eS10) to promote subunit recycling after translation stalling. Related references: PMID:31981475, PMID:34348161, PMID:34469731
• Subcellularly, USP10 localizes to the cytoplasm, nucleus, and early endosomes, consistent with its diverse functional roles.
• USP10 undergoes post-translational modifications including acetylation and phosphorylation, and alternative splicing generates multiple isoforms.
• It is widely expressed across tissues and is implicated in processes such as DNA repair, NF-kappa-B signaling attenuation, and negative regulation of RLR antiviral signaling.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (aa 1-100) of human USP10, which may contribute to recognizing the protein across isoforms. Validate specificity using knockdown or knockout controls.
• For Western blot, expect a band at ~87 kDa (predicted molecular weight). Optimize loading amounts and antibody dilution for your sample type.
• For IF/ICC, consider co-staining with markers for cytoplasm, nucleus, and endosomes to reflect USP10's multiple localizations.
• ELISA can be used for quantifying USP10 levels in cell lysates or recombinant protein; include appropriate standards and negative controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive one-stop sourcing partner for IVD raw materials, supporting diagnostic manufacturers, laboratories, and research institutions from concept to clinic. The USP10 Rabbit mAb targets a key regulator implicated in deubiquitination, autophagy, and DNA damage pathways, making it valuable for oncology and cell biology research. We supply this high-quality monoclonal antibody along with a full portfolio of validated antibody pairs, optimized bulk reagents, and rare-target raw material sourcing to streamline your assay development and procurement needs.