Uromodulin/UMOD Rabbit mAb for WB, IF-P, IHC-P, ELISA - P07911

Core Product Specifications and Parameters

Biological Background: Uromodulin Function and Localization

• Uromodulin (also known as Tamm-Horsfall urinary glycoprotein, THP) is the most abundant protein in normal human urine and is encoded by the UMOD gene.
• It contributes to the biogenesis and organization of the apical membrane in thick ascending limb of Henle's loop (TALH) epithelial cells, forming a gel-like structure that may influence water barrier permeability.
• Acts as a receptor for binding and endocytosis of cytokines (IL-1, IL-2) and TNF, linking it to renal immune responses. Related references: PMID:3498215
• Facilitates neutrophil migration across renal epithelia, potentially modulating inflammatory cell trafficking in the kidney. Related references: PMID:20798515
• Protects against urinary tract infections by binding to type 1 fimbriated E. coli via the bacterial adhesin fimH, promoting bacterial aggregation and preventing urothelial adhesion, with activity also against K. pneumoniae, P. aeruginosa, and S. mitis. Related references: PMID:11134021 PMID:32616672
• Localization includes apical and basolateral cell membranes, cilium membrane, and secretion into the urine, consistent with roles both at the cell surface and in the extracellular milieu.
• Expressed exclusively in kidney epithelial cells of the TALH and distal convoluted tubule, highlighting its specialized renal function.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the central region (aa 175–324) of human Uromodulin, which lies within the ZP domain and is conserved; consider validating reactivity in your species and sample type of interest.
• For WB, multiple bands may be observed (~55–115 kDa) due to glycosylation and proteolytic processing; include appropriate molecular weight markers and consider using deglycosylation controls if band pattern clarification is needed.
• This antibody is listed for WB, IF-P, IHC-P, and ELISA; for tissues or cells with low expression, optimize detection conditions and verify with relevant positive and negative controls.
• Cross-reactivity is reported for human and mouse; if using mouse models, confirm performance in your specific strain and tissue context.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio bridges diagnostic manufacturers and research institutes to a comprehensive portfolio of IVD raw materials, including validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target sourcing. This anti-Uromodulin rabbit monoclonal antibody supports research in renal physiology, urinary tract infection mechanisms, and kidney disease biomarker development. Leverage our integrated supply chain to accelerate your assay development from concept to clinical application.