UQCRH Rabbit mAb for WB, IHC-P, ELISA - P07919

Core Product Specifications and Parameters

Biological Background: UQCRH Function and Localization

• UQCRH (cytochrome b-c1 complex subunit 6, mitochondrial hinge protein, complex III subunit VIII) is a nuclear-encoded component of the mitochondrial respiratory chain complex III (ubiquinol-cytochrome c oxidoreductase).
• It is essential for the assembly and stability of complex III, which catalyzes electron transfer from ubiquinol to cytochrome c via the Q cycle, coupling this to proton translocation across the inner mitochondrial membrane to drive ATP synthesis.
• The UQCRH gene (Gene ID 7388) encodes a precursor protein; after mitochondrial import and cleavage of the transit peptide, the mature protein has a molecular weight of approximately 11 kDa.
• UQCRH localizes to the mitochondrial inner membrane as part of the multi-subunit complex III.
• The protein contains a structural disulfide bond and undergoes acetylation, post-translational modifications that may modulate its function or interactions.
• Mutations in UQCRH are linked to mitochondrial complex III deficiency, nuclear type 11 (MC3DN11), a primary mitochondrial disease.
• Keywords associated with UQCRH include: 3D-structure, Acetylation, Disulfide bond, Electron transport, Membrane, Mitochondrion, Mitochondrion inner membrane, Primary mitochondrial disease, Respiratory chain, Transit peptide, Transport.

Experimental Guidance and Technical Tips

• This rabbit monoclonal antibody was generated against a synthetic peptide from the N-terminal region (amino acids 1–91) of human UQCRH. Consider using positive control lysates from mitochondria-rich tissues (e.g., heart, skeletal muscle) or purified mitochondrial fractions.
• For Western blotting, expect a band at approximately 11 kDa. Due to the small size, a 15% SDS-PAGE gel and optimized low molecular weight transfer conditions are recommended.
• In immunohistochemistry (IHC-P), effective antigen retrieval and permeabilization may be needed for mitochondrial detection. Validate in your specific tissue or cell system.
• The antibody cross-reacts with mouse and rat UQCRH based on sequence homology; however, verification in your species and application is advised.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with comprehensive access to IVD raw materials, technical services, and consulting—supporting every stage from concept to clinic. This anti-UQCRH rabbit monoclonal antibody is an excellent tool for mitochondrial complex III studies and diagnostic development targeting respiratory chain proteins. Beyond single antibodies, CamelBio can supply validated antibody pairs, bulk ancillary reagents, and assist in sourcing rare targets to accelerate your IVD program.