UNC5C Rabbit Polyclonal Antibody for WB and ELISA - O95185

Core Product Specifications and Parameters

Biological Background: UNC5C Function and Localization

• UNC5C (Netrin receptor UNC5C), also known as UNC5H3, is a transmembrane receptor belonging to the UNC-5 family.
• It functions as a netrin-dependent axon guidance receptor, mediating repulsion of neuronal growth cones during nervous system development; NTN1/netrin-1 binding may dissociate UNC5C from polymerized TUBB3, increasing microtubule dynamics and axon repulsion. Related references: PMID:28483977
• UNC5C may collaborate with DSCAM in NTN1-mediated axon repulsion independently of DCC, and is involved in corticospinal tract axon guidance and dorsal root ganglion axon projection towards the spinal cord. Related references: PMID:28483977
• Acts as a dependence receptor, inducing apoptosis in the absence of netrin ligand.
• Subcellular localization includes the cell membrane, synapses, growth cones, lamellipodia, and filopodia, consistent with its role in axon guidance.
• Predominantly expressed in the brain, particularly in temporal lobe cortical neurons and hippocampal pyramidal layer. Also detected in kidney but not in developing or adult lung. Related references: PMID:25419706 PMID:9782087
• Post-translational modifications include glycosylation, phosphorylation, and disulfide bond formation, which may regulate its function.
• Associated with neurodegenerative processes such as Alzheimer disease and amyloidosis, as indicated by UniProt keywords.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 300-360, a region that may influence epitope accessibility; consider using a blocking peptide or competitive assay to confirm specificity.
• For WB, the predicted molecular weight is 103 kDa; however, due to glycosylation and potential cleavage, additional bands may be observed. Validate with positive controls from relevant tissues (e.g., brain lysates from human, mouse, or rat).
• In ELISA, optimize coating concentration and antibody dilution using purified recombinant protein or cell lysates with known UNC5C expression.
• Since UNC5C expression is tissue-specific, inclusion of appropriate negative controls (e.g., lung lysates) is recommended.
• As a polyclonal antibody, lot-to-lot variability is possible; always validate performance in your experimental system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with a comprehensive portfolio of IVD raw materials, including validated primary antibodies like this UNC5C polyclonal antibody. With expertise spanning antibody development, bulk supply, and technical consulting, CamelBio supports neuroscience and axon guidance research from early-stage discovery to clinical application. Contact us to explore custom sourcing solutions for rare targets and ancillary reagents.