ULBP2 Rabbit Polyclonal Antibody for WB and ELISA - Q9BZM5

Core Product Specifications and Parameters

Biological Background: ULBP2 Function and Localization

• ULBP2 (UL16-binding protein 2) is a GPI-anchored cell surface glycoprotein that functions as a stress-induced ligand for the activating receptor NKG2D (KLRK1) on natural killer cells and subsets of T cells.
• Binding of ULBP2 to NKG2D triggers cytotoxicity, thereby mediating innate immune surveillance against tumors and infected cells.
• ULBP2 is primarily expressed in a variety of cancer cell lines and fetal tissues, while being largely absent from normal adult tissues, highlighting its potential as a tumor marker or therapeutic target.
• The protein localizes to the cell membrane and endoplasmic reticulum, and a soluble secreted form has been detected, suggesting both contact-dependent and paracrine immunoregulatory functions.
• ULBP2 belongs to the RAET1 family of MHC class I-related molecules and is also known as ALCAN-alpha and retinoic acid early transcript 1H (RAET1H), indicating its regulation by retinoic acid.
• Post-translational modifications include N-linked glycosylation and a GPI anchor, and the protein features a signal peptide and intramolecular disulfide bonds, contributing to its structural stability.
• UniProt annotation includes keywords such as "Host-virus interaction" and "Immunity", indicating its relevance in viral immune evasion strategies; for example, human cytomegalovirus protein UL16 binds to ULBP2 to prevent NKG2D activation.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a peptide within the extracellular domain of ULBP2, which is predicted to be exposed in both membrane-bound and soluble forms, making this antibody suitable for detecting native and recombinant ULBP2 in applications such as Western blot and ELISA.
• For Western blot, use reducing conditions; ULBP2 migrates at approximately 27 kDa. Always validate the antibody in your specific sample system, as glycosylation may affect migration.
• In ELISA, consider pairing this antibody with a suitable matched pair for sandwich detection, though independent validation is recommended.
• Given the limited expression in normal tissues, appropriate positive (cancer cell lines) and negative (normal tissue lysates) controls should be included.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and researchers with comprehensive access to high-quality IVD raw materials. From validated antibody pairs and monoclonal/polyclonal antibodies to bulk ancillary reagents and rare target sourcing, we bridge the gap from concept to clinic. For your NKG2D ligand and tumor immunity projects, CamelBio offers a reliable supply chain and expert technical consultation.