UCP3 Rabbit pAb for WB, IHC-P, ELISA - P55916

Core Product Specifications and Parameters

Biological Background: UCP3 Function and Localization

• UCP3 (Uncoupling Protein 3), also designated SLC25A9, is a mitochondrial carrier protein originally proposed to mediate proton leak across the inner mitochondrial membrane, uncoupling ATP synthesis from electron transport. Related references: PMID:11171965, PMID:12670931, PMID:12734183
• Its function remains controversial, and recent evidence suggests UCP3 may primarily act as a metabolite transporter rather than a classical uncoupler. Related references: PMID:21775425, PMID:36114012, PMID:11707458
• The protein is localized to the mitochondrial inner membrane, a position consistent with its role in metabolite shuttling and potential mild uncoupling.
• Tissue expression is highly restricted; UCP3 is predominantly found in skeletal muscle, heart, and brown/white adipose tissue, with higher levels in glycolytic versus oxidative muscle fibers. Related references: PMID:9305858
• Alternative splicing generates multiple UCP3 isoforms, which may differ in activity or regulation.
• Genetic variants of UCP3 have been associated with metabolic disorders including obesity and diabetes mellitus.
• Key structural features include six transmembrane helices characteristic of the mitochondrial carrier family, and it is classified under transport-related keywords such as 'Transmembrane', 'Transmembrane helix', and 'Mitochondrion inner membrane'.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a recombinant fragment spanning amino acids 20–119 of human UCP3 (NP_073714.1); epitope information can assist in orthogonal validation.
• For Western blotting, a band near 34 kDa is expected; this predicted molecular weight should be used as a reference, but actual migration may vary depending on gel and buffer conditions.
• When applying IHC-P, consider optimizing antigen retrieval protocols and validate staining in tissues known to express UCP3, such as skeletal muscle or heart.
• As a polyclonal antibody, confirm lot-to-lot consistency by including a positive control sample (e.g., human muscle lysate) in each assay.
• Cross-reactivity with mouse and rat has been demonstrated, but verify sensitivity in your specific sample matrices.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio connects diagnostic manufacturers and research laboratories with high-quality IVD raw materials, including the UCP3 Rabbit pAb for mitochondrial metabolism studies. We offer validated antibody pairs, bulk monoclonal/polyclonal antibodies, and accessory reagents to streamline your assay development pipeline. For rare or specialized targets like UCP3, our team provides rapid sourcing support—contact us to discuss custom requirements and technical guidance.