Ubiquityl-Histone H2A-K119 (P04908/P0C0S8) Rabbit Polyclonal Antibody for WB and ELISA

Core Product Specifications and Parameters

Biological Background: Histone H2A Type 1 Function and Localization

Histone H2A Function

Histone H2A is a core component of the nucleosome, wrapping and compacting DNA into chromatin. This structural role limits DNA accessibility and thereby centrally regulates transcription, DNA repair, DNA replication, and chromosomal stability. DNA accessibility is modulated through a complex set of post-translational modifications (PTMs) known as the "histone code" and by nucleosome remodeling.

Subcellular Localization

Histone H2A localizes primarily to the nucleus and associates with chromosomes.

Post-Translational Modifications

Histone H2A undergoes extensive PTMs, including acetylation, phosphorylation, citrullination, and ubiquitination. Specific annotated modifications include:

• N-terminal acetylation at Ser-2.
• Phosphorylation at Ser-2 by RPS6KA5.
• Citrullination at Arg-4.
• At Lys-119, the residue targeted by this antibody, multiple acylations have been identified: hydroxyisobutyrylation, beta‑hydroxybutyrylation, crotonylation, and glutarylation. The keyword "Ubl conjugation" in UniProt supports that ubiquitination also occurs at this site.

Related references: PMID:11709551, PMID:15823041, PMID:16457589, PMID:15010469, PMID:24681537, PMID:27105115, PMID:21925322, PMID:31542297

Experimental Guidance and Technical Tips

• The immunogen is a synthetic ubiquitinated peptide around K119 of human HIST1H2AB (UniProt P04908/P0C0S8). This design suggests specificity for the ubiquitylated form; verify specificity using appropriate controls (e.g., comparison with unmodified peptide or lysates from cells with modulated ubiquitination).
• For Western blot (WB), consider extracting histones using acid extraction protocols to enrich for histone proteins. The predicted unmodified molecular weight is approximately 14 kDa; ubiquitination adds ~8.5 kDa, so a band around 23 kDa may be observed.
• For ELISA, direct or sandwich formats can be used. Consider coating with the ubiquitinated peptide or with recombinant ubiquitinated histone H2A to assess antibody binding.
• Given the wide predicted cross-reactivity (Human, Mouse, Rat, and others), validate the antibody in your specific sample system before scaling up.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research institutes with a one-stop sourcing solution for IVD raw materials, including validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents. This Ubiquityl-Histone H2A-K119 rabbit polyclonal antibody, targeting a key epigenetic mark, exemplifies our ability to supply rare modification-specific research reagents alongside comprehensive sourcing for histone biology and chromatin R&D applications.