UBE4A Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - Q14139

Core Product Specifications and Parameters

Biological Background: UBE4A Function and Localization

• UBE4A, also known as E4, UFD2, UBOX2, NEDHMS, is a ubiquitin conjugation factor E4 A that functions as a RING-type E3 ubiquitin transferase.
• It acts as an E3 ubiquitin ligase and may also serve as an E4 ligase, extending polyubiquitin chains on pre-ubiquitinated substrates.
• Mediates 'Lys-48'-linked polyubiquitination, a signal typically targeting proteins for proteasomal degradation.
• Localizes predominantly to the cytoplasm, where it participates in the ubiquitin-proteasome pathway.
• Undergoes post-translational modifications such as acetylation and is subject to alternative splicing, potentially generating multiple isoforms.
• Mutations in the UBE4A gene have been associated with intellectual disability, underscoring its importance in neuronal function.

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 797–1066 of human UBE4A; consider this region when assessing epitope specificity across species and isoforms.
• For Western blot (WB), UBE4A has a predicted molecular weight of ~123 kDa; ensure adequate gel separation and transfer conditions for high-molecular-weight proteins.
• When using immunohistochemistry (IHC-P), validate with appropriate tissue controls and optimize antigen retrieval protocols.
• Rabbit polyclonal antibodies offer robust signal amplification but may exhibit batch-to-batch variability; include positive and negative controls in each experiment.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio partners with diagnostic manufacturers and research laboratories to deliver a comprehensive range of IVD raw materials and technical support, from early-stage development through clinical application. This anti-UBE4A polyclonal antibody serves as a valuable tool for investigating ubiquitin-mediated proteolysis and disease-linked pathways. As a single-contact sourcing hub, we offer validated antibodies, bulk ancillary reagents, and custom procurement for rare or niche targets—streamlining your workflow and scaling your projects efficiently.