UBE2W Rabbit Polyclonal Antibody for WB, ELISA - Q96B02

Core Product Specifications and Parameters

Biological Background: UBE2W Function and Localization

• UBE2W (also known as UBC-16, UBC16) is an E2 ubiquitin-conjugating enzyme that functions by accepting ubiquitin from the E1 complex and catalyzing its covalent attachment to substrate proteins.
  Related references: PMID:20061386, PMID:21229326
• It specifically monoubiquitinates the N-terminus of various substrates, including ATXN3, MAPT/TAU, POLR2H/RPB8, and STUB1/CHIP, by recognizing backbone atoms of disordered N-termini.
  Related references: PMID:23560854, PMID:23696636, PMID:25436519
• UBE2W is involved in the degradation of misfolded chaperone substrates by mediating monoubiquitination of STUB1/CHIP, which recruits ATXN3 and restricts the length of ubiquitin chains attached to STUB1/CHIP substrates.
• It acts as a specific E2 enzyme for the Fanconi anemia complex after UV irradiation, associating with FANCL to monoubiquitinate FANCD2, a critical step in DNA damage repair.
  Related references: PMID:19111657, PMID:21229326
• UBE2W can catalyze 'Lys-11'-linked polyubiquitination in vitro and can function even in the absence of active E3 ligases, albeit at a slower rate.
  Related references: PMID:25436519
• Subcellular localization studies indicate that UBE2W is predominantly found in the nucleus.
• It is widely expressed across tissues, with highest expression in brain, liver, pancreas, and heart.

Experimental Guidance and Technical Tips

• The immunogen used is a recombinant fusion protein corresponding to amino acids 40-110 of human UBE2W, which may provide specificity for the human target; cross-reactivity with mouse has been observed, suggesting potential recognition of conserved epitopes.
• For Western blot analysis, a band around 17 kDa is expected based on the predicted molecular weight of UBE2W; pre-validated positive controls such as HeLa or HEK293 cell lysates may be suitable due to the ubiquitous expression of UBE2W.
• In ELISA applications, consider using recombinant UBE2W as a capture antigen and titrate the antibody to determine optimal working dilutions.
• Given the nuclear localization of UBE2W, effective nuclear extraction protocols may be necessary for optimal detection in cellular fractionation Western blots.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your dedicated one-stop sourcing partner for IVD raw materials, offering validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents. For researchers targeting the ubiquitin-proteasome pathway or DNA damage repair, CamelBio ensures reliable access to high-quality UBE2W antibodies and related reagents, streamlining your development from concept to clinic.