UBE2I Rabbit pAb for WB - P63279

Core Product Specifications and Parameters

Biological Background: UBE2I (UBC9) Function and Localization

• UBE2I, also known as SUMO-conjugating enzyme UBC9, ubiquitin carrier protein 9, or p18, is a key E2 enzyme in the SUMOylation pathway.
• It accepts SUMO proteins (SUMO1-4 and SUMO5) from the E1 complex and catalyzes their covalent attachment to target proteins, often with the help of E3 ligases like RANBP2 and CBX4.
• UBE2I is essential for nuclear architecture and chromosome segregation, playing a critical role in mitosis and cell cycle progression.
• It mediates sumoylation of ERCC6, which is necessary for transcription-coupled nucleotide excision repair. Related references: PMID:26620705
• UBE2I sumoylates SHMT1 at Lys-38/39, promoting its RAN-dependent nuclear import. Related references: PMID:17446168
• It also sumoylates TYMS and DHFR, linking SUMOylation to one-carbon metabolism. Related references: PMID:18067453
• The protein localizes to the nucleus, cytoplasm, and perinuclear region, consistent with its diverse substrate interactions.
• UBE2I is widely expressed across tissues including heart, skeletal muscle, pancreas, kidney, liver, lung, placenta, brain, testis, and thymus.

Experimental Guidance and Technical Tips

• The immunogen targets amino acids 1-158 of human UBE2I; this N-terminal region may allow detection of full-length UBE2I.
• For Western blot (WB), human, mouse, and rat cell lysates are suitable based on cross-reactivity.
• In IHC-P and IF/ICC, consider optimizing antigen retrieval and fixation conditions to preserve the epitope.
• Since UBE2I functions as a SUMO E2 enzyme, analyzing samples treated with SUMO pathway modulators may reveal dynamic changes.
• Pair this antibody with appropriate controls to confirm specificity for downstream ELISA or other quantitative assays.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research labs with reliable access to IVD raw materials including validated antibodies and ancillary reagents. This UBE2I polyclonal antibody supports studies in SUMOylation, cell cycle, and DNA repair. For bulk orders or custom sourcing of rare targets, partner with CamelBio.